Tuning the photoluminescence by engineering surface states/size of S, N co-doped carbon dots for cellular imaging applications
Tuning the photoluminescence by engineering surface states/size of S, N co-doped carbon dots for cellular imaging applications
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DOI：
10.1088/1361-6528/ac56f2
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发表时间：
2022-02
期刊：
影响因子：
3.5
通讯作者：
A. Sonsin;E. Silva;Aldilane L X Marques;Lais V A T Silva;Sendy M S Nascimento;S. T. Souza;A. Borbely;C.D.A.E.S. Barbosa;Eduardo J S Fonseca
中科院分区：
文献类型：
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作者：
A. Sonsin;E. Silva;Aldilane L X Marques;Lais V A T Silva;Sendy M S Nascimento;S. T. Souza;A. Borbely;C.D.A.E.S. Barbosa;Eduardo J S Fonseca
本研究以柠檬酸和半胱氨酸为碳源，采用水热法合成了氮硫共掺杂的碳量子点。系统研究了303-453 K固态热处理对CD的尺寸、表面、荧光和细胞毒性的影响。通过一个简单的STT，可以调整表面状态和CD的平均尺寸，从而导致永久的红移。最初，CD显示细胞活力随着浓度的增加而降低。然而，在STT后，其活力随着浓度的增加而保持恒定。在这里，我们展示了根据STT之前（蓝色发射）和之后（红色发射）的CD荧光发射来标记细胞质的可能性。本文研究的CD显示出选择性发光特性，这是任何细胞成像应用的基础。
In this research, we have synthesized carbon dots (CDs) co-doped with nitrogen and sulfur by facile hydrothermal method, using citric acid and cysteine as carbon source. The effect of solid-state thermic treatment (STT) at 303–453 K on the size, surface, fluorescence and cellular cytotoxicity of the CDs were systematically investigated. Through a simple STT, it was possible to tune surface states and the average size of the CDs, causing a permanent red shift. Initially, CDs showed a decrease in cell viability with increasing concentration. However, after STT, its viability remained constant with an increase in concentration. Here, we show the possibility to label the cells cytoplasm according to the CDs fluorescence emission before (blue emission) and after STT (red emission). The CDs studied in this paper show selective luminescence properties, which are fundamental for any cell imaging application.