COEXISTENCE OF SACCHAROMYCES-CEREVISIAE AND ESCHERICHIA-COLI IN CHEMOSTAT UNDER SUBSTRATE COMPETITION AND PRODUCT INHIBITION
COEXISTENCE OF SACCHAROMYCES-CEREVISIAE AND ESCHERICHIA-COLI IN CHEMOSTAT UNDER SUBSTRATE COMPETITION AND PRODUCT INHIBITION
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DOI：
10.1002/bit.260281119
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发表时间：
1986-11-01
影响因子：
3.8
通讯作者：
STEPHANOPOULOS, G
中科院分区：
文献类型：
--
作者：
DAVISON, BH;STEPHANOPOULOS, G
在恒定的操作条件下，在恒化器中建立了酿酒酵母和大肠杆菌的稳定共存稳态混合培养物。这些物种竞争葡萄糖，生长限制资源，并产生乙酸和乙醇。乙酸对E.大肠杆菌在pH 5的纯培养物中，而乙醇抑制仅是边缘的。对S.通过乙酸盐或乙醇观察到酿酒酵母生长。测量纯培养物参数并用于分析。当两种微生物都产生抑制剂时，线性稳定性分析表明，随着进料浓度降低，可能会出现三种稳定结果的过渡。实验研究验证了这些预测，并从酵母生长稳态，共存稳态，并在一个E。通过将料液中葡萄糖浓度从10 g/L降低到5 g/L和2.5 g/L，分别获得了大肠杆菌的生长稳定状态。这种动态行为与其他竞争抑制组合的结果不同，并首次通过实验证明，由于底物竞争和产物抑制，共存是可能的。
A mixed culture of Saccharomyces cerevisiae and Escherichia coli was established in a stable coexistence steady state in a chemostat under constant operating conditions. The species competed for glucose, the growth-limiting resource, and produced acetate and ethanol. The acetic acid was shown to be very inhibitory to E. coli in pure culture at pH 5 while ethanol inhibition was only marginal. No significant inhibition of S. cerevisiae growth was observed by either acetate or ethanol. Pure culture parameters were measured and used in the analysis. Linearized stability analysis for the case when both organisms produce the inhibitor showed that the transition through three stable outcomes was possible as the feed concentration is lowered. Experimental studies verified these predictions, and successive transitions from a yeast growth steady state, to a coexistence steady state, and to an E. coli growth steady state were obtained by lowering the glucose concentration in the feed from 10 to 5 to 2.5 g/L, respectively. This dynamic behavior is distinct from the outcomes of other competition-inhibition combinations and experimentally demonstrates for the first time that coexistence is possible due to substrate competition and product inhibition.