Preparation of fully bio-based multilayers composed of heparin-like carboxymethylcellulose sodium and chitosan to functionalize poly (l-lactic acid) film for cardiovascular implant applications.
Preparation of fully bio-based multilayers composed of heparin-like carboxymethylcellulose sodium and chitosan to functionalize poly (l-lactic acid) film for cardiovascular implant applications.
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DOI：
10.1016/j.ijbiomac.2023.123285
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发表时间：
2023-01
影响因子：
8.2
通讯作者：
Kun Wang;Ying Yu;Wei Li;Da Li;Hui Li
中科院分区：
文献类型：
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作者：
Kun Wang;Ying Yu;Wei Li;Da Li;Hui Li
本研究以羧甲基纤维素钠（CMC）为原料，通过硫酸酯化制备肝素样多糖，然后通过层层组装（LBL）的方法在聚乳酸（PLA）表面组装成由硫酸酯化羧甲基纤维素钠（SCMC）和壳聚糖（CS）组成的生物双层膜，用于制备生物可吸收血管支架等与血液接触的材料。对不同硫酸化程度的SCMC进行了初步结构和生物活性研究，筛选出性能最佳的SCMC。采用X射线光电子能谱仪、扫描电镜和水接触角测定仪研究了SCMC/CS多层膜改性PLA膜的表面化学组成、形貌和润湿性。对SCMC/CS多层膜改性PLA膜进行了一系列抗凝血试验。与未改性PLA膜相比，（SCMC/CS）15多层改性PLA膜的蛋白质吸附量和平板粘附力分别降低了44.6%和71.5%，活化部分凝血活酶时间延长了11.9倍，凝血酶时间超过300 s，接触活化率和溶血率显著降低。此外，改性后的聚乳酸膜对L929成纤维细胞具有良好的细胞相容性，并具有良好的抗炎和抗菌能力。结论：SCMC/CS多层改性聚乳酸膜具有良好的血液相容性、细胞相容性、抗炎性和抗菌性，具有良好的临床应用前景。
In this study, heparin-like polysaccharides were successfully produced by sulfation of carboxymethylcellulose sodium, then a fully biobased bilayer composed of sulfated carboxymethylcellulose sodium (SCMC) and chitosan (CS) was composited on the surface of Poly (L-lactic acid) (PLA) through layer-by-layer (LBL) assembly for the potential blood-contact application such as bioresorbable vascular scaffold. The preliminary structure and bioactivity of SCMC with different degree of sulfation were investigated, and the SCMC with best performance was selected. The surface chemical compositions, morphologies and wettability of SCMC/CS multilayer-modified PLA films were researched by X-ray photoelectron spectrometer, scanning electron microscopy and water contact angle meter. A series of anticoagulation tests of SCMC/CS multilayer-modified PLA films were performed. In term of (SCMC/CS)15multilayer-modified PLA film, the protein adsorption and plate adhesion decreased by 44.6 % and 71.5 %, respectively, the activated partial thromboplastin time prolonged by 11.9 times and thrombin time exceed 300 s, the contact activation and hemolysis rate significantly reduced compared with unmodified PLA film. Besides, this modified PLA films performed good cytocompatibility to L929 fibroblast cells, excellent anti-inflammatory and antibacterial abilities. In conclusion, the multifunctional SCMC/CS multilayer-modified PLA films with hemocompatibility, cytocompatibility, anti-inflammatory and antibacterial properties may have promising potential in future clinical applications.