Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein
Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein
复制标题
茉莉酸调节拟南芥 PIN2 蛋白的内吞作用和质膜积累
DOI：
10.1111/j.1469-8137.2011.03713.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Li, Chuanyou
中科院分区：
文献类型：
--
作者：
Sun, Jiaqiang;Chen, Qian;Li, Chuanyou
生长素转运蛋白PIN- formed （PIN）家族的亚细胞分布在生长素梯度介导的发育过程中起着关键作用，包括侧根形成和向地生长。在这里，我们报道了CORONATINE INSENSITIVE 1 （COI1）和AUXIN RESISTANT 1 （AXR1）依赖性茉莉酸甲酯（MeJA）对PIN2亚细胞分布的两个不同方面的影响：在较低浓度（5 μ M）下，MeJA抑制PIN2的内噬作用，而在较高浓度（50 μ M）下，MeJA减少PIN2在质膜中的积累。我们发现ASA1 （ANTHRANILATE SYNTHASE a1）和TIR1/AFBs （TRANSPORT INHIBITOR RESPONSE 1/生长素信号F-BOX PROTEINs）生长素受体基因的突变削弱了5 μ M MeJA对PIN2内吞作用的抑制作用，表明较低浓度的茉莉酸盐通过与生长素途径相互作用抑制PIN2内吞作用。相反，ASA1和TIR1/AFBs生长素受体基因的突变增强而不是削弱了50 μ M MeJA对质膜上PIN2积累的减少作用，这表明茉莉酸的这种作用是独立于生长素途径的。除了MeJA对PIN2内吞作用和质膜驻留的影响外，我们还发现MeJA改变了重力刺激下生长素的侧向再分配，从而损害了根的向地性反应。我们的研究结果强调了茉莉素-生长素相互作用在协调植物生长和适应反应中的重要性。
The subcellular distribution of the PIN-FORMED (PIN) family of auxin transporters plays a critical role in auxin gradient-mediated developmental processes, including lateral root formation and gravitropic growth.Here, we report two distinct aspects of CORONATINE INSENSITIVE 1 (COI1)-and AUXIN RESISTANT 1 (AXR1)-dependent methyl jasmonate (MeJA) effects on PIN2 subcellular distribution: at lower concentration (5 mu M), MeJA inhibits PIN2 endocytosis, whereas, at higher concentration (50 mu M), MeJA reduces PIN2 accumulation in the plasma membrane.We show that mutations of ASA1 (ANTHRANILATE SYNTHASE a1) and the TIR1/AFBs (TRANSPORT INHIBITOR RESPONSE 1/AUXIN-SIGNALING F-BOX PROTEINs) auxin receptor genes impair the inhibitory effect of 5 mu M MeJA on PIN2 endocytosis, suggesting that a lower concentration of jasmonate inhibits PIN2 endocytosis through interaction with the auxin pathway. In contrast, mutations of ASA1 and the TIR1/AFBs auxin receptor genes enhance, rather than impair, the reduction effect of 50 mu M MeJA on the plasma membrane accumulation of PIN2, suggesting that this action of jasmonate is independent of the auxin pathway. In addition to the MeJA effects on PIN2 endocytosis and plasma membrane residence, we also show that MeJA alters lateral auxin redistribution on gravi-stimulation, and therefore impairs the root gravitropic response.Our results highlight the importance of jasmonate-auxin interaction in the coordination of plant growth and the adaptation response.