CULTURED MAMMALIAN-CELLS ATTACH TO THE INVASIN PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS
CULTURED MAMMALIAN-CELLS ATTACH TO THE INVASIN PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS
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DOI：
10.1073/pnas.85.18.6682
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
LEONG, JM
中科院分区：
文献类型：
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作者：
ISBERG, RR;LEONG, JM
侵袭素（假结核耶尔森氏菌 inv 基因的产物）的表达允许肠道细菌进入培养的哺乳动物细胞。研究了侵入素结合动物细胞的能力以及这种相互作用在进入过程中的潜在意义。研究发现，HEp-2 细胞可以附着在涂有含有入侵素的细菌膜的表面上。通过在 NaDodSO4/聚丙烯酰胺凝胶上分级分离细菌膜蛋白并将蛋白质转移至过滤器，我们证明了膜的细胞结合成分与侵袭素共迁移。改变蛋白质电泳迁移率的突变也引起细胞结合活性迁移的相应变化，表明共迁移蛋白质确实是侵袭素。针对 invasin 的单克隆抗体可阻断 invasin-HEp-2 细胞相互作用，也可抑制细菌穿透 HEp-2 细胞，表明该蛋白质与动物细胞的相互作用对于细胞渗透至关重要。
The expression of invasin, the product of the Yersinia pseudotuberculosis inv gene, allows enteric bacteria to enter cultured mammalian cells. The ability of invasin to bind animal cells and the potential significance of this interaction in the entry process were investigated. It was found that HEp-2 cells could attach to surfaces coated with bacterial membranes containing invasin. By fractionating bacterial membrane proteins on NaDodSO4/polyacrylamide gels and transferring the protein to filters, we demonstrated that the cell-binding component of the membranes comigrated with invasin. Mutations that changed the electrophoretic mobility of the protein also caused a corresponding shift in the migration of the cell-binding activity, showing that the comigrating protein was indeed invasin. Monoclonal antibodies directed against invasin that blocked invasin-HEp-2 cell interaction also inhibited bacteria from penetrating HEp-2 cells, indicating that interaction of this protein with animal cells is critical for cellular penetration.