Chlorantraniliprole Resistance in the Diamondback Moth (Lepidoptera: Plutellidae)
Chlorantraniliprole Resistance in the Diamondback Moth (Lepidoptera: Plutellidae)
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小菜蛾（鳞翅目：小菜蛾科）对氯虫苯甲酰胺的抗性
DOI：
10.1603/ec13456
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发表时间：
2014-04-01
影响因子：
2.2
通讯作者：
Xue, Chao-Bin
中科院分区：
文献类型：
--
作者：
Gong, Wei;Yan, Hui-Hui;Xue, Chao-Bin
选择性靶向昆虫ryanodine受体（RyR）的氯虫苯甲酰胺在防治小菜蛾Plutella xylostella（L.）导致对该杀虫剂的抗性发展日益突出。虽然对RyR的结构和功能已经进行了大量的研究，但小菜蛾对氯虫苯甲酰胺抗性的分子机制仍需进一步研究。通过室内筛选和田间采集，获得了对氯虫苯甲酰胺具有中、高抗性的小菜蛾菌株。测定了氯虫苯甲酰胺对敏感品系和抗性品系3龄幼虫的生物活性，并对其抗性发展和生物适合度进行了研究。抗性的实现遗传力（h（2））表明小菜蛾对氯虫苯甲酰胺具有较高的抗性风险。抗性品系中RyR基因的表达水平低于敏感品系，表明PxRyR基因表达的降低可能与小菜蛾对氯虫苯甲酰胺的抗性有关。从四个菌株（S、F-18、BY和ZC）中克隆并鉴定了RyR cDNA的4，400 bp片段，其编码RyR的大部分功能结构域。在三个耐药菌株（F-18、BY和ZC）中发现了14个氨基酸（Q（4546）DS 4559）的缺失。如先前发表的文章中所报道的，在两种耐药菌株（BY和ZC）的羧基末端区域也发现了导致甘氨酸至谷氨酸取代的点突变。这些结果表明，RyR基因转录水平的降低和该位点的突变可能与小菜蛾对氯虫苯甲酰胺的抗性有关。
The wide application of chlorantraniliprole, which selectively targets insect ryanodine receptors (RyR), for control of the diamondback moth, Plutella xylostella (L.), has led to increasingly prominent development of resistance to this insecticide. Although much work has been carried out on the structure and function of RyR, the molecular mechanisms of resistance to chlorantraniliprole in diamondback moth still needs further investigation. P. xylostella strains with medium and high resistance to chlorantraniliprole were obtained by laboratory selection and field collection. The biological activity of chlorantraniliprole against the third-instar larvae of susceptible and resistant strains was tested, and resistance development and biological fitness were investigated. The realized heritability (h(2)) of resistance showed the diamondback moth has a high risk of resistance to chlorantraniliprole. RyR transcript levels were lower in resistant strains than in susceptible strains, indicating that decreased expression of PxRyR may be associated with chlorantraniliprole resistance in P. xylostella. A 4,400 bp fragment of the RyR cDNA, which encodes most of the functional domains of RyR, was cloned and characterized from four strains (S, F-18, BY, and ZC). A 14 amino acid (Q(4546) DS4559) deletion was found in three resistant strains (F-18, BY, and ZC). A point mutation resulting in a glycine to glutamate substitution, as reported in a previously published article, was also found in the carboxyl-terminal region of two resistant strains (BY and ZC). These results indicated that decreased transcriptional level of RyR mRNA and combined with the site mutation might be related to chlorantraniliprole resistance in P. xylostella.