Microelectrode array stimulation combined with intrinsic optical imaging: A novel tool for functional brain mapping.
Microelectrode array stimulation combined with intrinsic optical imaging: A novel tool for functional brain mapping.
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DOI：
10.1016/j.jneumeth.2016.01.018
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发表时间：
2016-04-01
影响因子：
3
通讯作者：
Roe AW
中科院分区：
文献类型：
--
作者：
Chernov MM;Chen G;Torre-Healy LA;Friedman RM;Roe AW
皮层微刺激脑功能地形图是一种广泛使用的临床和实验工具。然而，传统上数据是逐点收集的，这使得这项技术非常耗时。此外，即使在熟练的人手中，也很难实现始终如一的渗透深度。最后，对微刺激的效果进行了行为学评估，没有尝试捕捉被刺激的局部皮质回路的活动。我们提出了一种结合微电极阵列和本征光学成像的脑功能地形图的新方法。电极的精确间距允许快速、准确地在规则网格中绘制感兴趣区域的地图。同时，当刺激与本征光学成像相结合时，光学窗口允许对局部神经连接进行可视化。我们使用灵长类运动皮质作为样本应用，结合清醒和麻醉状态下的微刺激、成像和电生理记录，展示了我们的技术的有效性。我们发现，用我们的方法收集的数据与其他人发表的数据是一致的。我们认为，我们的方法能够以更快和更一致的方式收集数据，并使一些用传统方法难以进行的研究成为可能。我们的技术允许在清醒的受试者中在多个会话中同时调制和成像皮质感觉运动网络，这对于皮层组织的研究和脑机接口的设计都是非常理想的。
Functional brain mapping via cortical microstimulation is a widely used clinical and experimental tool. However, data are traditionally collected point by point, making the technique very time consuming. Moreover, even in skilled hands, consistent penetration depths are difficult to achieve. Finally, the effects of microstimulation are assessed behaviorally, with no attempt to capture the activity of the local cortical circuits being stimulated. We propose a novel method for functional brain mapping, which combines the use of a microelectrode array with intrinsic optical imaging. The precise spacing of electrodes allows for fast, accurate mapping of the area of interest in a regular grid. At the same time, the optical window allows for visualization of local neural connections when stimulation is combined with intrinsic optical imaging. We demonstrate the efficacy of our technique using the primate motor cortex as a sample application, using a combination of microstimulation, imaging and electrophysiological recordings during wakefulness and under anesthesia. We find the data collected with our method is consistent with previous data published by others. We believe that our approach enables data to be collected faster and in a more consistent fashion and makes possible a number of studies that would be difficult to carry out with the traditional approach. Our technique allows for simultaneous modulation and imaging of cortical sensorimotor networks in wakeful subjects over multiple sessions which is highly desirable for both the study of cortical organization and the design of brain machine interfaces.