Effects of surface pre-reacted glass-ionomer fillers on mineral induction by phosphoprotein
Effects of surface pre-reacted glass-ionomer fillers on mineral induction by phosphoprotein
复制标题
DOI：
10.1016/j.jdent.2010.10.011
复制
发表时间：
2011-01-01
影响因子：
4.4
通讯作者：
Saito, Takashi
中科院分区：
文献类型：
--
作者：
Ito, Shuichi;Iijima, Masahiro;Saito, Takashi
目的：研究表面预反应玻璃离子（S-PRG）填料释放的离子在体外磷蛋白诱导下的矿化电位。方法：以牙本质磷蛋白为模型。用二乙烯基砜将磷维素固定在琼脂糖珠上。五等分磷维素固定化琼脂糖珠在对照或实验矿化液中孵育。实验矿化溶液由树脂洗脱液填充S-PRG填料制成。在37℃的摇晃水浴中孵育，并在孵育期间的几个时间点取等量。然后用原子吸收光谱法分析微珠的钙含量。结果：光维素固定化琼脂糖珠在亚稳溶液中培养5.3 h后诱导了矿物的形成，而不需要从S-PRG填料中洗脱离子。未稀释的洗脱液显著缩短了矿物诱导时间。扫描电镜观察和x射线衍射显示，与S-PRG填料洗脱液孵育的珠子上的磷灰石晶体比对照大。结论：S-PRG填充物可能在矿物诱导中起一定作用。(C) 2010 Elsevier Ltd.版权所有。
Objectives: The aim of this study was to evaluate the mineralizing potential of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers on mineral induction by phosphoprotein in vitro.Methods: Phosvitin was used as a model of dentin phosphoprotein in this study. Phosvitin was immobilized on agarose beads with divinyl sulfone. Five aliquots of phosvitin-immobilized agarose beads were incubated in control or experimental mineralizing solution. The experimental mineralizing solutions were made from eluates of resin filled with S-PRG fillers. The beads were incubated at 37 degrees C in a shaking water bath, and aliquots were taken at several time points during the incubation. Then the beads were analyzed for calcium by atomic absorption spectrometry.Results: Phosvitin-immobilized agarose beads induced mineral formation after incubation for 5.3 h in the metastable solution without ions eluted from S-PRG fillers. Undiluted eluates significantly reduced mineral induction time. SEM observation and X-ray diffraction revealed larger apatite crystals on the beads incubated with eluates of S-PRG fillers than with the control.Conclusions: S-PRG fillers may play a role in mineral induction. (C) 2010 Elsevier Ltd. All rights reserved.