Curcumin-carrying nanoparticles prevent ischemia-reperfusion injury in human renal cells.
Curcumin-carrying nanoparticles prevent ischemia-reperfusion injury in human renal cells.
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携带姜黄素的纳米颗粒可预防人肾细胞缺血再灌注损伤
DOI：
10.18632/oncotarget.13626
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发表时间：
2016-12-27
期刊：
影响因子：
--
通讯作者：
Zheng DH
中科院分区：
文献类型：
--
作者：
Xu Y;Hu N;Jiang W;Yuan HF;Zheng DH
肾缺血再灌注损伤(IRI)是临床上的主要并发症。然而，尽管这种情况经常发生，但有效的预防/治疗策略很少。姜黄素具有抗氧化作用，是一种很有潜力的肾脏IRI保护剂，但其较差的水溶性限制了其应用。在本研究中，我们构建了姜黄素载distearoylphosphatidylethanolamine-polyethylene乙二醇纳米粒(CuR-NPs)，并在体外检测了它们对再灌注损伤HK-2细胞的影响。纳米粒包裹的姜黄素具有更好的水溶性和缓释作用。与IRI组和姜黄素组相比，Cur-NP组细胞存活率显著提高，caspase-3和Bax蛋白表达下调，Bcl2蛋白表达上调，抗氧化超氧化物歧化酶水平升高，细胞凋亡率、活性氧水平和丙二醛含量降低。结果表明，CuR-NPs具有良好的水溶性和缓释性，对IRI所致的HK-2细胞具有保护作用。
Renal ischemia-reperfusion injury (IRI) is a major complication in clinical practice. However, despite its frequency, effective preventive/treatment strategies for this condition are scarce. Curcumin possesses antioxidant properties and is a promising potential protective agent against renal IRI, but its poor water solubility restricts its application. In this study, we constructed curcumin-carrying distearoylphosphatidylethanolamine-polyethylene glycol nanoparticles (Cur-NPs), and their effect on HK-2 cells exposed to IRI was examined in vitro. Curcumin encapsulated in NPs demonstrated improved water solubility and slowed release. Compared with the IRI and Curcumin groups, Cur-NP groups displayed significantly improved cell viability, downregulated protein expression levels of caspase-3 and Bax, upregulated expression of Bcl-2 protein, increased antioxidant superoxide dismutase level, and reduced apoptotic rate, reactive oxygen species level, and malondialdehyde content. Results clearly showed that Cur-NPs demonstrated good water solubility and slow release, as well as exerted protective effects against oxidative stress in cultured HK-2 cells exposed to IRI.