Lateral Flow Immunoassay
Lateral Flow Immunoassay
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DOI：
10.1007/978-1-4939-2742-5_13
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Ching, Kathryn H.
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文献类型：
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作者：
Ching, Kathryn H.
侧流免疫测定（LFIAs）是快速诊断领域中的主要方法。这些小型手持设备不需要专门的培训或设备来操作，并在样品应用的几分钟内产生结果。对于许多类型的家庭检测试剂盒、紧急救援人员以及寻求快速评估给定样本的食品制造商和生产商来说，它们是理想的形式。LFIA依赖于高质量的单克隆抗体，可识别目标分析物。随着单克隆抗体技术越来越容易被小型实验室所利用，人们对开发LFIA原型用于潜在的商业生产的兴趣越来越大。在本章中，将介绍设计和构建LFIA原型的基础知识。
Lateral flow immunoassays (LFIAs) are a staple in the field of rapid diagnostics. These small handheld devices require no specialized training or equipment to operate, and generate a result within minutes of sample application. They are an ideal format for many types of home test kits, for emergency responders and for food manufacturers and producers looking for a quick evaluation of a given sample. LFIAs rely on high quality monoclonal antibodies that recognize the analyte of interest. As monoclonal antibody technology becomes more accessible to smaller laboratories, there has been increased interest in developing LFIA prototypes for potential commercial manufacture. In this chapter, the basics of designing and building an LFIA prototype are described.