Association of VCAM-1 overexpression with oncogenesis, tumor angiogenesis and metastasis of gastric carcinoma
Association of VCAM-1 overexpression with oncogenesis, tumor angiogenesis and metastasis of gastric carcinoma
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DOI：
10.3748/wjg.v9.i7.1409
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发表时间：
2003-07-01
影响因子：
4.3
通讯作者：
Yang, L
中科院分区：
文献类型：
--
作者：
Ding, YB;Chen, GY;Yang, L
目的：探讨血管细胞粘附分子-1（VCAM-1）的表达与胃癌发生、肿瘤血管生成及转移的关系，并评价血清VCAM-1水平在胃癌中的临床意义。方法：检测41例胃癌患者、8例良性胃溃疡患者和10例健康受试者标本中VCAM-1的表达。 通过免疫组织化学检测 VCAM-1。通过在 x200 放大倍数下对用单克隆抗体 CD34 免疫染色的内皮细胞进行计数来测量微血管密度 (MVD)。通过酶联免疫吸附测定法测量了 41 名胃癌患者术前和术后 7 天以及 25 名健康对照者的血清 VCAM-1 浓度。评估术前血清 VCAM-1 水平与临床病理特征及其术后变化之间的关联。另外还检测了血清癌胚抗原(CEA)。结果：41例胃癌组织中，VCAM-1阳性31例(75.6%)。 VCAM-1 阳性胃癌比 VCAM-1 阴性胃癌更具侵袭性并且处于更晚期。 VCAM-1 阳性癌症比 VCAM-1 阴性癌症与更多淋巴结转移相关（P
AIM: To investigate the relationship between the expression of vascular cell adhesion molecule-1 (VCAM-1) and oncogenesis, tumor angiogenesis and metastasis in gastric carcinoma, and to evaluate the clinical significance of serum VCAM-1 levels in gastric cancer.METHODS: Specimens from 41 patients with gastric cancer, 8 patients with benign gastric ulcer, and 10 healthy subjects were detected for the expression of VCAM-1 by immunohistochemistry. Microvessel density (MVD) was measured by counting the endothelial cells immunostained with the monoclonal antibody CD34 at x200 magnification. Serum VCAM-1 concentrations were measured by an enzyme linked immunosorbent assay in the 41 gastric cancer patients before surgery and at 7 days after surgery as well as in 25 healthy controls. The association between preoperative serum VCAM-1 levels and clinicopathological features, and their changes following surgery was evaluated. In addition, serum carcinoembryonic antigen (CEA) was also examined.RESULTS: Of the 41 gastric cancer tissues, 31 (75.6 %) were VCAM-1 positive. The VCAM-1 positive gastric cancers were more invasive and classified in the more advanced stage than the VCAM-1 negative ones. The VCAM-1 positive cancers were associated with more lymph node metastases than VCAM-1-negative ones (P