Patterns of peroxidative ethane emission from submerged rice seedlings indicate that damage from reactive oxygen species takes place during submergence and is not necessarily a post-anoxic phenomenon
Patterns of peroxidative ethane emission from submerged rice seedlings indicate that damage from reactive oxygen species takes place during submergence and is not necessarily a post-anoxic phenomenon
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浸没水稻秧苗的过氧化乙烷排放模式表明，活性氧的破坏发生在浸没期间，不一定是缺氧后的现象
DOI：
10.1007/s00425-006-0457-z
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发表时间：
2007-06-01
期刊：
影响因子：
4.3
通讯作者：
Harren, F. J. M.
中科院分区：
文献类型：
--
作者：
Santosa, I. E.;Ram, P. C.;Harren, F. J. M.
以乙烷作为活性氧（ROS）过氧化损伤膜的标记物，研究了水稻幼苗在黑暗条件下淹水过程中受到的伤害。通常认为，由ROS引起的膜损伤是在浸没诱导的缺氧后重新引入氧气时发生的浸没后现象。我们发现，乙烷的生产，从水稻幼苗淹没24-72小时，刺激4-37 nl gFW（-1），表明水下膜过氧化。一周后检查时，幼苗受损或死亡。在空气中脱泡时，乙烷产率急剧上升，但在2小时后回落至小于0.1 nl gFW（-1）h（-1）。我们比较了淹涝敏感和耐淹品种，淹涝开始在上午（更多的损害）和下午（较少的损害），并调查了不同的淹涝持续时间。当乙烷排放总量超过15 nl gFW（-1）时，幼苗表现出广泛的死亡。少量的乙烷排放与对叶片的广泛伤害较小有关。在气相混合物中施加2 h的部分缺氧（O-2水平< 1%）也刺激乙烷产生。相反，幼苗在厌氧气相条件下不产生乙烷，直到重新充气：然后观察到一个小的峰值，随后是一个低的，稳定的乙烷生产。我们的结论是，在淹没过程中的损害是不相关的广泛缺氧。相反，伤害与幼苗中的膜过氧化有关，这些幼苗在淹没时部分缺氧。在返回到空气中，进一步的过氧化反应被抑制在约2小时内，表明有效的控制ROS生产过程中不明显的淹没本身。
Using ethane as a marker for peroxidative damage to membranes by reactive oxygen species (ROS) we examined the injury of rice seedlings during submergence in the dark. It is often expressed that membrane injury from ROS is a post-submergence phenomenon occurring when oxygen is re-introduced after submergence-induced anoxia. We found that ethane production, from rice seedlings submerged for 24-72 h, was stimulated to 4-37 nl gFW(-1), indicating underwater membrane peroxidation. When examined a week later the seedlings were damaged or had died. On de-submergence in air, ethane production rates rose sharply, but fell back to less than 0.1 nl gFW(-1) h(-1) after 2 h. We compared submergence-susceptible and submergence-tolerant cultivars, submergence starting in the morning (more damage) and in the afternoon (less damage) and investigated different submergence durations. The seedlings showed extensive fatality whenever total ethane emission exceeded about 15 nl gFW(-1). Smaller amounts of ethane emission were linked to less extensive injury to leaves. Partial oxygen shortage (O-2 levels < 1%) imposed for 2 h in gas phase mixtures also stimulated ethane production. In contrast, seedlings under anaerobic gas phase conditions produced no ethane until re-aerated: then a small peak was observed followed by a low, steady ethane production. We conclude that damage during submergence is not associated with extensive anoxia. Instead, injury is linked to membrane peroxidation in seedlings that are partially oxygen deficient while submerged. On return to air, further peroxidation is suppressed within about 2 h indicating effective control of ROS production not evident during submergence itself.