Construction of a yeast mutant lacking the mitochondrial nuclease.
Construction of a yeast mutant lacking the mitochondrial nuclease.
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构建缺乏线粒体核酸酶的酵母突变体。
DOI：
10.1093/nar/16.8.3283
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发表时间：
1988
影响因子：
14.9
通讯作者：
Zona,M
中科院分区：
文献类型：
--
作者：
Zassenhaus,HP;Hofmann,TJ;Uthayashanker,R;Vincent,RD;Zona,M
克隆了编码主要线粒体核酸酶的酿酒酵母核基因。通过线粒体核酸酶特异性抗体从λGT11文库中鉴定基因序列。用噬菌体重组体的DNA从质粒库中分离出整个核酸酶基因。多拷贝质粒载体上含有核酸酶基因的酵母菌株的线粒体核酸酶产量是野生型菌株的20-40倍。含有核酸酶基因零等位基因的菌株缺乏所有线粒体核酸酶的痕迹。然而，这两种细胞类型都是表型野生型，这表明核酸酶1S不是线粒体功能的必需酶。编码线粒体核酸酶的基因被称为NUC1。
The nuclear gene fromSaccharomyces cerevisiaethat encodes the major mitochondrial nuclease was cloned. Gene sequences were identified from a λgtll library by antibodies specific to the mitochondrial nuclease. DNA fror the phage recombinant was used to isolate the entire nuclease gene from a plasmid library. Yeast strains containing the nuclease gene on a multicopy plasmid vector overproduced mitochondrial nuclease 20–40 times relative to a wild-type strain. Strains containing a null allele of the nuclease gene lacked all traces of mitochondrial nuclease. Both cell types, however, were phenotypically wild-type indicating that the nuclease 1s not an essential enzyme for mitochondrial function. The locus encoding the mitochondrial nuclease is termedNUC1.