Photo-Dimerization Induced Dynamic Viscoelastic Changes in ABA Triblock Copolymer-Based Hydrogels for 3D Cell Culture
Photo-Dimerization Induced Dynamic Viscoelastic Changes in ABA Triblock Copolymer-Based Hydrogels for 3D Cell Culture
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DOI：
10.1021/acs.chemmater.6b02839
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发表时间：
2016-09-13
影响因子：
8.6
通讯作者：
Yoshida, Ryo
中科院分区：
文献类型：
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作者：
Tamate, Ryota;Ueki, Takeshi;Yoshida, Ryo
细胞不仅能感知生化信号，还能对生物物理信号(如基质弹性)作出反应。除了基质弹性，最近的研究表明，粘弹性(如应力松弛)显著影响细胞功能，如铺展、增殖和分化。在此，我们描述了一种细胞相容性和动态水凝胶，它由定义明确的ABA三嵌段共聚物组成，仅通过紫外光照射就能显著改变粘弹性：A块包含N-异丙基丙烯酰胺和带有香豆素侧链的丙烯酸酯单体，而B块是生物相容性聚环氧乙烷。三嵌段共聚物在生理条件下形成物理交联的水凝胶。然而，在紫外光照射下)，由于香豆素部分的光二聚作用，水凝胶的粘弹性是动态调节的。紫外光照射后，水凝胶的流变性发生了很大的变化，说明水凝胶中形成了化学交联点。最后，我们证明了水凝胶粘弹性的动态变化对微囊化细胞的行为有显著的影响。
Cells sense and respond not only to biochemical signals but also to biophysical signals (e.g., matrix elasticity). In addition to matrix elasticity, recent studies have revealed that viscoelasticity (e.g., stress relaxation) significantly affects cellular functions, such as spreading, proliferation, and differentiation, Herein) we describe a cytocompatible and dynamic hydrogel that is comprised of well-defined ABA triblock copolymers, which enable significant changes of viscoelastic properties solely by UV light irradiation: The A block contains N-isopropylacrylamide and an acrylate monomer with a coumarin side chain, whereas the B block is biocompatible poly(ethylene oxide). The triblock copolymer forms a physically cross-linked hydrogel under physiologically relevant conditions. However, under UV light irradiation) the viscoelasticity of the hydrogel is dynamically modulated due to the photodimerization of coumarin moieties. After UV illumination, rheological properties of the hydrogel are drastically different, indicating the formation of chemical cross-linking points in the hydrogel. Finally, we demonstrate that the dynamic change in the viscoelasticity of the hydrogel has, a significant influence on the behavior of encapsulated cells.