East Asian Passiflora distortion virus: a novel potyvirus species causing deformation of passionfruits in Japan
East Asian Passiflora distortion virus: a novel potyvirus species causing deformation of passionfruits in Japan
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东亚西番莲变形病毒：一种在日本引起西番莲果变形的新型马铃薯病毒属
DOI：
10.1007/s10327-019-00842-0
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发表时间：
2019
影响因子：
1.2
通讯作者：
Riska・Yuka Sato・Kanami Inudo・Masayuki Nakamura・Tomohiro Fukumoto・Tetsuya Takushi・Shin-ichi Fuji・Hisashi Iwai
中科院分区：
文献类型：
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作者：
清水茜;大熊祐之介;片岡創;小林括平;山岡直人;八丈野孝;Riska・Yuka Sato・Kanami Inudo・Masayuki Nakamura・Tomohiro Fukumoto・Tetsuya Takushi・Shin-ichi Fuji・Hisashi Iwai
在2013年从秋田县秋田市、2014年从冲绳县名护市、2015年从鹿儿岛县萨摩町和与龙岛采集的具有花叶、卷叶和果实畸形的西番莲植株中检测到假定属于新马铃薯Y病毒的病毒。针对在日本引起西番莲木质化病害的东亚西番莲病毒（EAPV）-AO（Amami Oshima株系）的ELISA和RT-PCR未检测到该病毒的分离物。将分离株命名为PV-AK（秋田）、PV-OK（冲绳）、PV-YO（Yoron）和PV-SA（萨摩）。在13个菜豆品种的寄主范围试验中，4个被PV分离物系统感染，2个被EAPV-AO系统感染。豇豆黑穗病三种株系被PV分离物系统感染，并在远离接种叶片的未接种叶片上诱导花叶症状，但对EAPV-AO免疫。结果表明，该病毒基因组全长9973个核苷酸（不包括poly（A）尾），编码3217个氨基酸的多聚蛋白，两侧为129-130个核苷酸的5′非编码区和193个核苷酸的3′非编码区。全基因组核苷酸和氨基酸序列分析表明，它们属于同一种。与PV分离物具有最高全基因组核苷酸同一性的马铃薯Y病毒是西瓜花叶病毒，其共享68.1%的同一性，而EAPV-AO为65.3%。根据ICTV命名规则，这些PV分离物属于Potyvirusgenus中的一个新种，但与EAPV有较远的亲缘关系。在此，我们建议将东亚西番莲扭曲病毒（East Asian Passiflora Distortion Virus）作为新的种名。
Viruses assumed to belong to a newPotyviruswere detected in passionfruit plants with foliar mosaic, leaf curl and fruit malformation that were collected from Akita City, Akita Prefecture in 2013, Nago City, Okinawa Prefecture in 2014, and Satsuma Town and Yoron Island, Kagoshima Prefecture in 2015. The isolates of this virus species were not detected by ELISA and RT-PCR targeting East Asian Passiflora virus (EAPV)-AO (Amami Oshima strain), which induces woodiness disease of passionfruit in Japan. The isolates were designated PV-AK (Akita), PV-OK (Okinawa), PV-YO (Yoron), and PV-SA (Satsuma). In a host-range test of 13 cultivars of French bean, four were systemically infected with the PV isolates and two with EAPV-AO. Cowpea cv. Kurodane sanjaku was systemically infected with PV isolates and induced mosaic symptoms on non-inoculated leaves distant from the inoculated leaves but was immune to EAPV-AO. The complete genomic sequences of the PV isolates consisted of 9973 nucleotides (nt) excluding the poly(A) tail, and encoded 3217 amino acids of polyprotein, flanked by 129–130 nt of the 5′-noncoding region and 193 nt of the 3′-noncoding region. Whole-genome nucleotide and amino acid sequences indicated that they belong to identical species. The potyvirus with the highest whole-genome nucleotide identity to the PV isolates is watermelon mosaic virus, which shares 68.1% identity, versus 65.3% for EAPV-AO. On the basis of ICTV-naming regulations, these PV isolates belong to a new species in thePotyvirusgenus, but are distantly related to EAPV. Here, we propose East Asian Passiflora distortion virus as the new species name.