Effect of Cell Association on in vitro Chondrogenesis of Mesenchyme Cells from Quail Limb Buds
Effect of Cell Association on in vitro Chondrogenesis of Mesenchyme Cells from Quail Limb Buds
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细胞联合对鹌鹑肢芽间充质细胞体外软骨形成的影响
DOI：
10.1247/csf.5.239
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发表时间：
1980
影响因子：
1.5
通讯作者：
Y. Kuroda
中科院分区：
文献类型：
--
作者：
E. Matsutani;Y. Kuroda
与鹌鹑胚胎的肢体芽（第21-22阶段）分离的间充质细胞用于研究各个时期不同类型的细胞缔合对软骨分化的影响。首先将间充质细胞作为单层，沉淀的颗粒或带回旋摇动或相互振动的培养物孵育4-48 h。然后将细胞解离，并检查其在低细胞密度下在培养物中形成软骨菌落的能力。被孵育为单层或具有相互振动的培养物的细胞仅形成成纤维细胞菌落，而在伴有旋转摇动的培养物中孵育12小时或作为沉淀16小时形成软骨菌落的细胞。在最后两种处理中，根据先前的孵育期，软骨菌落的数量增加。因此，在一定时间段内的三维细胞缔合可能会稳定间充质细胞分化为软骨细胞的能力。
Mesenchyme cells dissociated from the limb buds of quail embryos (stages 21-22) were used to study the effects of different types of cell association for various periods on cartilage differentiation. The mesenchyme cells were first incubated for 4-48 h as monolayers, precipitated pellets, or cultures with gyratory shaking or reciprocal shaking. The cells then were dissociated, and their ability to form cartilage colonies in cultures at low cell density was examined. Cells that had been incubated as monolayers or in cultures with reciprocal shaking formed only fibroblastic colonies, whereas cells that had been incubated in cultures with gyratory shaking for 12 h or as pellets for 16 h formed cartilage colonies. With the last two treatments, the number of cartilage colonies increased according to the prior incubation period. Thus, three-dimensional cell association for a certain period may stabi-lize the ability of mesenchyme cells to differentiate into cartilage cells.