Central gustatory lesions and learned taste aversions: Unconditioned stimuli
Central gustatory lesions and learned taste aversions: Unconditioned stimuli
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DOI：
10.1016/j.physbeh.2005.12.008
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发表时间：
2006-03-30
影响因子：
2.9
通讯作者：
Norgren, R
中科院分区：
文献类型：
--
作者：
Mungarndee, SS;Lundy, RF;Norgren, R
用鹅膏蕈氨酸（ibotenic acid，IBO）损毁大鼠丘脑味觉核（tastatory nucleus of thalamus，TTAx）和臂旁内侧核（medial and lateral parabracial nucleus，mPBNx，IPBNx），观察两种不同的非条件刺激（unconditioned stimulation，US）对条件性味觉厌恶（conditioned taste aversion，CTA）的影响。最初的研究确定了两种US的等效剂量，LiCl和环磷酰胺（CY），使用非病变大鼠。随后，使用一组单独的损伤动物及其共享对照（SHAM），将CY注射与两种味觉刺激（CS）之一配对3次，每组中一半大鼠注射0.1 M NaCl，另一半注射0.2 M蔗糖。在这些调节试验之后，CS在没有US的情况下再呈现两次，首先在1瓶测试中，然后在2瓶选择中用水。采集和测试试验有2个干预性仅用水日，以确保完全补液。两周后，再次测试相同的大鼠，以获得CTA，使用LiCl作为US和与CY试验期间使用的CS相反的CS。SHAM和TTAx组学会了避免食用与CY或LiCl治疗相关的味道。这两个PBNx组没有学会厌恶，不管美国。(c)2006年爱思唯尔公司All rights reserved.
The efficacy of two different unconditioned stimuli (US) in producing conditioned taste aversion (CTA) was tested in rats after bilateral ibotenic acid (IBO) lesions of the gustatory nucleus of thalamus (TTAx) and the medial and lateral parabrachial nuclei (mPBNx, IPBNx). An initial study determined an equivalent dose for the two USs, LiCl and cyclophosphamide (CY), using non-lesioned rats. Subsequently, using a separate set of lesioned animals and their share controls (SHAM), injections of CY were paired 3 times with one of two taste stimuli (CSs), 0.1 M NaCl for half the rats in each group, 0.2 M sucrose for the other half. After these conditioning trials, the CS was presented twice more without the US, first in a 1-bottle test, then in a 2-bottle choice with water. The acquisition and test trials had 2 intervening water-only days to assure complete rehydration. Two weeks later, the same rats were tested again for acquisition of a CTA using LiCI as the US and the opposite CS as that used during the CY trials. The SHAM and TTAx groups learned to avoid consuming the taste associated with either CY or LiCl treatment. The two PBNx groups failed to learn an aversion regardless of the US. (c) 2006 Elsevier Inc. All rights reserved.