Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly (N-isopropylacrylamide) temperature-responsive surfaces.
Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly (N-isopropylacrylamide) temperature-responsive surfaces.
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DOI：
10.1002/jbm.1225
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
T. Collier;James M. Anderson;A. Kikuchi;T. Okano
中科院分区：
文献类型：
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作者：
T. Collier;James M. Anderson;A. Kikuchi;T. Okano
单核细胞和巨噬细胞的黏附和异物巨细胞(FBGC)的形成在具有广泛性质的表面上被观察到。在这项研究中，我们利用具有动态表面属性的新型温度响应性表面(TRS)来研究炎性细胞的黏附行为。随着温度的变化，接枝的聚-N-异丙基丙烯酰胺链通过它们较低的临界溶液温度(LCST)，并且可以延伸(水合物)，在20℃时形成亲水表面，或者收缩(脱水)，在37℃时产生疏水表面。当温度低于临界溶液温度时，细胞表面的润湿性发生变化，形成亲水性表面，贴壁细胞的差异性脱落随时间延长而减少，从培养2 h的98%下降到第10天的30%。这些贴壁的细胞仍然存活，并在TCPS上再培养3、7和10天。这些新的表面允许以时间的方式研究黏附性炎症细胞的黏附行为，以及表面构象和润湿性变化对细胞黏附和脱离的影响。
Monocyte and macrophage adhesion and foreign body giant cell (FBGC) formation has been observed on surfaces with a wide range of properties. In this study we have utilized novel, temperature-responsive surfaces (TRS) with dynamic surface properties to investigate inflammatory cell adhesion behavior. With temperature changes, grafted chains of poly-N-isopropylacrylamide pass through their lower critical solution temperature (LCST) and can either extend (hydrate), creating a hydrophilic surface at 20 degrees C, or contract (dehydrate), creating a hydrophobic surface at 37 degrees C. Isolated human monocytes and monocyte-derived macrophages were able to adhere, spread, and form FBGC on the hydrophobic surface. Decreasing the temperature below the lower critical solution temperature induced a change in the surface wettability, creating a hydrophilic surface, that induced a differential detachment of adherent cells that decreased with time, ranging from 98% after 2 h of culture to 30% at day 10. These detached cells remained viable, and were recultured onto TCPS for 3, 7, and 10 days. These novel surfaces allow investigation of the adhesive behavior of adherent inflammatory cells in a temporal manner, and the effects of surface conformation and wettability changes on cell adhesion and detachment.