Human primary bronchial epithelial cells respond differently to titanium dioxide nanoparticles than the lung epithelial cell lines A549 and BEAS-2B
Human primary bronchial epithelial cells respond differently to titanium dioxide nanoparticles than the lung epithelial cell lines A549 and BEAS-2B
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DOI：
10.3109/17435390.2011.598245
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发表时间：
2012-09-01
期刊：
影响因子：
5
通讯作者：
Bucht, Anders
中科院分区：
文献类型：
--
作者：
Ekstrand-Hammarstrom, Barbro;Akfur, Christine M.;Bucht, Anders
我们比较了正常人支气管上皮 (NHBE) 细胞和上皮细胞系（A549 和 BEAS-2B）对五种纳米晶二氧化钛 (TiO2) 制剂的细胞摄取和反应。 P25 纳米粒子同时含有锐钛矿和金红石修饰，可在所用的所有三种细胞类型中诱导活性氧 (ROS) 和中性粒细胞趋化剂 IL-8 的分泌。纯锐钛矿和金红石颗粒在 A549 中引起不同的 IL-8 反应，而在 BEAS-2B 细胞中没有反应，尽管 ROS 的形成相似。纯 TiO2 修饰还引发了 NHBE 细胞中炎症介质的释放：IL-6、G-CSF 和 VEGF，但在两种细胞系中则没有。我们得出的结论是，肺上皮细胞的反应性很大程度上取决于 TiO2 纳米粒子的理化性质和反应细胞的类型。在评估吸入纳米颗粒的不良反应时，应考虑原代肺上皮细胞与永生化细胞系的不同促炎反应性。
We have compared the cellular uptake and responses of five preparations of nanocrystalline titanium dioxide (TiO2) between normal human bronchial epithelial (NHBE) cells and epithelial cell lines (A549 and BEAS-2B). The P25 nanoparticles, containing both anatase and rutile modifications, induced reactive oxygen species (ROS) and secretion of the neutrophil chemoattractant IL-8 in all three cell types used. Pure anatase and rutile particles provoked differential IL-8 response in A549 and no response in BEAS-2B cells despite similar formation of ROS. The pure TiO2 modifications also provoked release of the inflammatory mediators: IL-6, G-CSF and VEGF, in NHBE cells but not in the two cell lines. We conclude that the responsiveness of lung epithelial cells is strongly dependent on both the physicochemical properties of TiO2 nanoparticles and the type of responder cells. The differential pro-inflammatory responsiveness of primary lung epithelial cells compared with immortalized cell lines should be considered in the assessment of adverse reactions to inhaled nanoparticles.