The presynaptic active zone protein Bassoon as a marker for synapses between Type III cells and afferent nerve fibers in taste buds
The presynaptic active zone protein Bassoon as a marker for synapses between Type III cells and afferent nerve fibers in taste buds
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突触前活性区蛋白巴松管作为 III 型细胞和味蕾传入神经纤维之间突触的标记
DOI：
10.1093/chemse/bjac016
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发表时间：
2022
期刊：
影响因子：
3.5
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Ikuta R.;Hamada;S.
味蕾是味觉的感受器。味蕾中有两种味觉感受器细胞：II型和III型细胞。III型细胞通过传统的化学突触与传入纤维相连。在本研究中，我们用免疫细胞化学方法研究了细胞骨架蛋白Bason在小鼠味蕾中常规突触活动区的分布模式。在III型细胞中，巴松管主要被检测到为小点状。在宝石内神经纤维附近或与之部分重叠处可观察到巴松管免疫反应阳性斑点。巴松管在味蕾中的分布格局在环状、菌状和叶状三种味蕾中相似。免疫电子显微镜显示环状味蕾中III型细胞常规突触的活动区有巴松管。目前的结果表明，巴松管是小鼠味蕾中III型细胞与传入纤维之间突触的标志。
Taste buds are receptor organs for gustation. Two types of taste receptor cells have been identified in taste buds: Type II and Type III cells. Type III cells connect with afferent fibers through conventional chemical synapses. In the present study, we used immunocytochemistry to examine the distribution pattern of Bassoon, a scaffolding protein of the cytomatrix at the active zones of conventional synapses in mouse taste buds. Bassoon was predominantly detected as small puncta in Type III cells. Bassoon-immunoreactive puncta were observed in proximity to or partially overlapping with intragemmal nerve fibers. The distribution pattern of Bassoon in taste buds was similar among circumvallate, fungiform, and foliate taste buds. Immunoelectron microscopy showed Bassoon at the active zones of the conventional synapses of Type III cells in circumvallate taste buds. The present results demonstrate that Bassoon is a marker for synapses between Type III cells and afferent fibers in mouse taste buds.