LRG1 May Accelerate the Progression of ccRCC via the TGF-β Pathway
LRG1 May Accelerate the Progression of ccRCC via the TGF-β Pathway
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LRG1 可能通过 TGF-β 途径加速 ccRCC 的进展
DOI：
10.1155/2020/1285068
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发表时间：
2020-03-30
影响因子：
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通讯作者：
Cai, Guangyan
中科院分区：
文献类型：
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作者：
Hong, Quan;Wang, Shuqiang;Cai, Guangyan
透明细胞肾细胞癌（ccRCC）占肾细胞癌（RCC）病例的60% - 70%。为晚期ccRCC寻找更多治疗靶点是一项紧迫任务。富含亮氨酸的α - 2 - 糖蛋白1（LRG1）是一种与多种恶性肿瘤相关的分泌蛋白。我们基于癌症基因组图谱（TCGA）的数据，聚焦于LRG1在ccRCC中的表达及作用机制，并进行了初步验证，包括LRG1表达检测、LRG1基因甲基化检测以及下游信号检测。我们发现，LRG1在ccRCC肾组织样本中过表达，且ccRCC中LRG1基因的甲基化水平显著降低。此外，LRG1的表达与患者生存率呈负相关。基于我们之前的研究以及本文所报道的验证结果，我们提出，去甲基化诱导的LRG1过表达很可能通过转化生长因子-β（TGF-β）通路加速ccRCC的进展。
Clear cell renal cell carcinoma (ccRCC) accounts for 60-70% of renal cell carcinoma (RCC) cases. It is an urgent mission to find more therapeutic targets for advanced ccRCC. Leucine-rich a-2-glycoprotein 1 (LRG1) is a secreted protein associated with a variety of malignancies. Our study focused on the expression and mechanism of LRG1 in ccRCC based on data from The Cancer Genome Atlas (TCGA) and provided primary verification including LRG1 expression detection, LRG1 gene methylation detection, and downstream signaling detection. We found that LRG1 was overexpressed in ccRCC kidney tissue samples, and the methylation level of LRG1 gene was significantly decreased in ccRCC. Moreover, the expression of LRG1 was negatively related to patient survival. Based on our previous study and the verification reported in this article, we propose that demethylation-induced overexpression of LRG1 is likely to accelerate ccRCC progression via the TGF-β pathway.