A comparative Raman and CARS imaging study of colon tissue
A comparative Raman and CARS imaging study of colon tissue
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DOI：
10.1002/jbio.200810063
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Krafft, Christoph;Ramoji, Anuradha A.;Popp, Juergen
两个互补的技术，线性拉曼和相干反斯托克斯拉曼散射（汽车）显微镜的信息内容的实验评估。汽车是拉曼光谱的一种非线性变体，与激光扫描显微镜相结合，可以在几秒钟内快速获取图像。从2850、1660、1450和1000 cm（-1）处的薄结肠组织切片记录汽车图像，并与拉曼图像进行比较。拉曼图像从单变量和多变量（k-均值聚类）方法获得，而所有汽车图像代表单变量结果。组织切片内的差异可以在汽车和拉曼图像的化学图中可视化。然而，识别组织类型和表征不同组织切片之间的差异，只有可能通过分析聚类平均光谱，从k-均值聚类分析。第一次比较为进一步开发汽车技术以评估组织奠定了基础。(C)WILEYNCH Verlag GmbH & Co. KGaA，魏因海姆
An experimental evaluation of the information content of two complimentary techniques, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy, is presented. CARS is a nonlinear variant of Raman spectroscopy that enables rapid acquisition of images within seconds in combination with laser scanning microscopes. CARS images were recorded from thin colon tissue sections at 2850, 1660, 1450 and 1000 cm(-1) and compared with Raman images. Raman images were obtained from univariate and multivariate (k-means clustering) methods, whereas all CARS images represent univariate results. Variances within tissue sections could be visualized in chemical maps of CARS and Raman imagas. However, identification of tissue types and characterization of variances between different tissue sections were only possible by analysis of cluster mean spectra, obtained from k-means cluster analysis. This first comparison establishes the foundation for further development of the CARS technology to assess tissue. (C) 2009 by WILEYNCH Verlag GmbH & Co. KGaA, Weinheim