Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system
Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system
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DOI：
10.1016/j.biomaterials.2012.07.023
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发表时间：
2012-11-01
期刊：
影响因子：
14
通讯作者：
Yoo, James J.
中科院分区：
文献类型：
--
作者：
Sullivan, David C.;Mirmalek-Sani, Sayed-Hadi;Yoo, James J.
终末期肾功能衰竭是一种毁灭性的疾病，供体器官移植是唯一的功能性恢复治疗。目前的供体器官数量满足不到五分之一的需求，因此再生医学方法已被提议作为潜在的治疗替代方案。一种这样的用于整个大器官生物工程的方法是将联合收割机功能性肾细胞与脱细胞猪肾支架结合。细胞去除的功效和保存的猪基质的生物相容性，以及支架的可重复性，对于这种方法的成功至关重要。我们评价了0.25%和0.5%十二烷基硫酸钠（SDS）和1% Triton X-100在成年猪肾脏脱细胞中的有效性。为了进行脱细胞，设计并构建了高通量系统。在这项研究中，所有三种方法都显示出显着的细胞去除，但0.5%SDS是最有效的去污剂（
End-stage renal failure is a devastating disease, with donor organ transplantation as the only functional restorative treatment. The current number of donor organs meets less than one-fifth of demand, so regenerative medicine approaches have been proposed as potential therapeutic alternatives. One such approach for whole large-organ bioengineering is to combine functional renal cells with a decellularized porcine kidney scaffold. The efficacy of cellular removal and biocompatibility of the preserved porcine matrices, as well as scaffold reproducibility, are critical to the success of this approach. We evaluated the effectiveness of 0.25 and 0.5% sodium dodecyl sulfate (SDS) and 1% Triton X-100 in the decellularization of adult porcine kidneys. To perform the decellularization, a high-throughput system was designed and constructed. In this study all three methods examined showed significant cellular removal, but 0.5% SDS was the most effective detergent (