Repeated ethanol administration induces short- and long-term changes in enkephalin and dynorphin tissue concentrations in rat brain
Repeated ethanol administration induces short- and long-term changes in enkephalin and dynorphin tissue concentrations in rat brain
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DOI：
10.1016/s0741-8329(00)00118-x
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发表时间：
2000-11-01
期刊：
影响因子：
2.3
通讯作者：
Nylander, I
中科院分区：
文献类型：
--
作者：
Lindholm, S;Ploj, K;Nylander, I
最近，我们已经表明，大鼠反复加热与乙醇和/或可卡因降低kappa -阿片受体mRNA水平的中脑边缘系统。本研究的目的是研究短期和长期的影响，重复乙醇给药对阿片肽的浓度在雄性Sprague-Dawley大鼠脑组织。强啡肽B（1-13）（Dyn B）和甲硫氨酸脑啡肽Arg（6）Phe（7）（MEAP）分别是κ和δ阿片受体的内源性配体，采用放射免疫分析法进行测定。连续13天给予大鼠乙醇（腹腔内注射），每日两次，2 g/kg bw/剂量]或生理盐水。第13天末次乙醇给药后30分钟，扣带回皮质中的Dyn B组织浓度显著降低。与盐水处理的对照组相比，最后一次乙醇注射后5天海马中的MEAP组织浓度降低。此外，在该时间点，导水管周围灰质区（PAG）中的Dyn B和MEAP浓度增加。特别令人感兴趣的是，在最后一次乙醇给药后30分钟和21天，在延髓核（NAcc）中发现Dyn B组织浓度显著增加。研究结果表明，重复的乙醇给药会诱导与动机和奖励相关的某些大脑区域中阿片类药物组织浓度的短期和长期变化。(C)2001 Elsevier Science Inc. All rights reserved.
Recently, we have shown that rats repeatedly heated with ethanol and/or cocaine have decreased kappa -opioid receptor mRNA levels in the mesolimbic system. The aim of the present study was to investigate the short- and long-term effects of repeated ethanol administration on opioid peptide concentrations in brain tissue of male Sprague-Dawley rats. Dynorphin B (1-13) (Dyn B) and Met-enkephalinArg(6)Phe(7) (MEAP), endogenous ligands to kappa- and delta -opioid receptors, respectively, were measured using radioimmunoassays. The rats were given either ethanol [intraperitoneal tip), twice daily, 2 g/kg bw/dose] or saline for 13 consecutive days. Thirty min after the last ethanol dose on Day 13, the Dyn B tissue concentration was significantly decreased in the cingulate cortex. The MEAP tissue concentration was decreased in the hippocampus 5 days after the last ethanol injection as compared to saline-treated controls. Furthermore, the Dyn B and the MEAP concentrations were increased in the periaqueductal grey area (PAG) at this time point. Of particular interest were the significant increases in Dyn B tissue concentrations found in the nucleus accumbens (NAcc) at 30 min and at 21 days after the last ethanol dose. The results suggest that repeated ethanol administration induces both short- and long-term changes in the tissue concentrations of opioids in certain brain regions associated with motivation and reward. (C) 2001 Elsevier Science Inc. All rights reserved.