Accumulation of glial fibrillary acidic protein in Müller radial glia during retinal degeneration.
Accumulation of glial fibrillary acidic protein in Müller radial glia during retinal degeneration.
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发表时间：
1988-09
影响因子：
4.4
通讯作者：
P. Ekström;S. Sanyal;K. Narfström;G. Chader;T. Veen
中科院分区：
文献类型：
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作者：
P. Ekström;S. Sanyal;K. Narfström;G. Chader;T. Veen
Müller放射状胶质细胞在视网膜损伤后积累胶质细胞酸性蛋白（GFAP）。我们已经研究了在遗传引起的视网膜营养不良的猫和小鼠品系中GFAP的细胞定位的变化：阿比西尼亚猫进行性视网膜营养不良，和小鼠视网膜变性（rd）和视网膜变性慢（rds）基因的纯合子和杂合子。本实验观察到：（1）正常猫和小鼠视网膜中存在胶质细胞酸性蛋白免疫反应（GFAP-IR）放射状Müller胶质细胞。(2)遗传性视网膜营养不良的猫和小鼠视网膜中GFAP-IR放射状Müller胶质细胞和其他GFAP-IR成分的数量随着年龄的增长而普遍增加。(3)在猫和小鼠中，GFAP积聚的Müller细胞的增加似乎从外周向中央视网膜进行。这可能反映了感光细胞退化的方向，在视网膜营养不良的猫和携带rds基因的小鼠中，感光细胞退化的方向是相同的。然而，在rd小鼠中，光感受器变性从中央到外周视网膜进行，表明GFAP积累不是光感受器损伤的局部直接效应。(4)比较不同突变品系小鼠，GFAP-IR元件的分布似乎存在质的差异。在+/+ rds/rds视网膜中，内丛状层和核层中的切线成分和纤维缠结的数量最多，其次是+/+ rds/+，而这些成分在rd/rd rds/rds中似乎更稀少，其次是rd/rd +/+和+/+ +/+视网膜。（250字处删节）
Müller radial glia accumulate glial fibrillary acid protein (GFAP) in response to retinal injuries. We have studied the changes in cellular localization of GFAP in genetically caused retinal dystrophy in strains of cat and mouse: Abyssinian cats with progressive retinal dystrophy, and mice homo- and heterozygous for the retinal degeneration (rd) and retinal degeneration slow (rds) genes. The following observations were made: (1) Glial fibrillary acid protein-immunoreactive (GFAP-IR) radial Müller glia are present in normal cat and mouse retinae. (2) There is a general increase with age in numbers of GFAP-IR radial Müller glia, and other GFAP-IR elements in the retina of cat and mice with hereditary retinal dystrophy. (3) The increase in GFAP-accumulating Müller cells seems to proceed from peripheral to central retina in both cats and mice. This might reflect the direction of photoreceptor degeneration, which proceeds in the same direction in the retinal dystrophic cat and in mice bearing the rds gene. However, in the rd mouse photoreceptor degeneration proceeds from central to peripheral retina, indicating that GFAP accumulation is not a local direct effect of photoreceptor damage. (4) Comparing the different mutant mouse strains, there seem to be qualitative differences in the distribution of GFAP-IR elements. The numbers of tangential elements and fibrillary tangles in the inner plexiform and nuclear layers are highest in the +/+ rds/rds retina, followed by the +/+ rds/+, whereas such elements appear to be more scarce in rd/rd rds/rds, followed by the rd/rd +/+ and +/+ +/+ retinae.(ABSTRACT TRUNCATED AT 250 WORDS)