Mitochondrial DNA typing of laser-captured single sperm cells to differentiate individuals in a mixed semen stain
Mitochondrial DNA typing of laser-captured single sperm cells to differentiate individuals in a mixed semen stain
复制标题
对激光捕获的单个精子细胞进行线粒体 DNA 分型，以区分混合精液染色中的个体
DOI：
10.1002/elps.201600009
复制
发表时间：
2016
期刊：
影响因子：
2.9
通讯作者：
王保捷
中科院分区：
文献类型：
--
作者：
张璐;丁梅;庞灏;邢佳鑫;宣金锋;王春红;林子清;韩松;梁克伟;李春梅;姚军;王保捷
两种精液混合样本的个体鉴定通常涉及常染色体和Y染色体短串联重复序列（STR）的分析，可以排除无亲缘关系的个体，但不能达到个体鉴定的目的。在精子细胞中，线粒体dna （mtDNA）有多个拷贝，在不同的母系相关个体中表现出遗传多态性。单细胞捕获技术可以在混合精液样本中获得一些单个精子细胞，然后利用聚合酶链反应从每个细胞中扩增mtDNA高变区I （HVR I）。通过汇集具有相同HVR I序列的细胞，我们可以获得足够的STR分型核DNA。
The identification of individuals in a mixture of two semen samples usually involves an analysis of autosomal and Y chromosomal short tandem repeats (STR) which can exclude unrelated individuals but cannot achieve the purpose of individual identification. In sperm cells, there are multiple copies of mitochondrial DNAs (mtDNA) which exhibit genetic polymorphisms in different matrilineal‐related individuals. Single‐cell capture technology can be applied to obtain some single sperm cells in a mixed semen sample, then polymerase chain reaction can be employed to amplify the mtDNA hypervariable region I (HVR I) from each cell. By pooling the cells with the same HVR I sequence, we can obtain the sufficient nuclear DNA for STR typing.