Immunodetection of estrogen receptor in epithelial and stromal tissues of neonatal mouse uterus.
Immunodetection of estrogen receptor in epithelial and stromal tissues of neonatal mouse uterus.
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新生小鼠子宫上皮和基质组织中雌激素受体的免疫检测。
DOI：
10.1073/pnas.85.10.3334
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发表时间：
1988
影响因子：
11.1
通讯作者：
John A. McLachlan
中科院分区：
文献类型：
--
作者：
K. Korach;Tsuneyoshi Horigome;Yasuhiro Tomooka;Shuji Yamashita;R. Newbold;John A. McLachlan
通过温和酶处理分离的上皮和基质部分测定新生小鼠子宫组织中雌激素受体的组织分布和水平。通过凝胶电泳分离分离级分的蛋白质，并使用单克隆抗体 H-222 在免疫印迹上检测受体。在 5 日龄和 10 日龄小鼠的生殖道组织样本中可检测到雌激素受体蛋白。 5 日龄动物上皮细胞中每单位 DNA 的受体水平低于基质中的受体水平。用己烯雌酚治疗后，两种组织类型的受体水平均增加，但黄体酮治疗后则没有。这些新生儿组织中存在的受体蛋白能够结合类固醇，如他莫昔芬氮丙啶亲和标记所证明的那样。对 4 日龄和 10 日龄小鼠子宫切片进行的免疫组织化学证实了生化结果，并表明上皮细胞中的核应变低于 4 日龄小鼠子宫基质细胞中的核应变。这些结果表明，雌激素受体蛋白存在于新生小鼠子宫的上皮和基质中，但基质细胞中的水平较高。
The tissue distribution and levels of estrogen receptor in neonatal mouse uterine tissue were determined in epithelial and stromal fractions separated by mild enzymatic treatment. Proteins of the isolated fractions were separated by gel electrophoresis and receptor was detected on immunoblots with monoclonal antibody H-222. Estrogen receptor protein was detectable in samples of reproductive tract tissue from 5- and 10-day-old mice. The level of receptor in 5-day-old animals was lower per unit DNA in epithelial cells than in stroma. Receptor levels were increased in both tissue types after treatment with diethylstilbestrol, but not with progesterone. Receptor protein present in these neonatal tissues was able to bind steroid as evidenced by affinity labeling with tamoxifen aziridine. Immunohistochemistry on sections of uteri from 4- and 10-day-old mice confirmed the biochemical results and indicated lower nuclear straining in epithelial cells than in stromal cells of uteri of 4-day-old mice. These results demonstrated that estrogen receptor protein is present in both epithelium and stroma of the neonatal mouse uterus, but at a higher level in stromal cells.