The distribution of betaglycan protein and mRNA in rat brain, pituitary, and gonads: implications for a role for betaglycan in inhibin-mediated reproductive functions.
The distribution of betaglycan protein and mRNA in rat brain, pituitary, and gonads: implications for a role for betaglycan in inhibin-mediated reproductive functions.
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DOI：
10.1210/endo.143.3.8707
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发表时间：
2002-03
期刊：
影响因子：
4.8
通讯作者：
L. Macconell;A. Leal;W. Vale
中科院分区：
文献类型：
--
作者：
L. Macconell;A. Leal;W. Vale
我们的实验室报道β聚糖作为一种β受体结合蛋白，并促进激活素信号传导的拮抗作用。虽然是TGF β和β受体的辅助受体，但其在生殖组织中的分布仍在很大程度上未被探索。组织化学分析显示β聚糖蛋白和mRNA分布在整个大鼠生殖轴。在脑中，β聚糖mRNA定位于前脑和脑干的离散区域，包括嗅核、隔核和下丘脑核。在垂体中，在前叶和中叶中观察到中等水平的β聚糖蛋白和mRNA。β-聚糖免疫反应性与所有垂体细胞亚型共定位，在最大程度上与促性腺激素的人口。在性腺中，β聚糖mRNA定位于细胞隔室中，与其蛋白质的大部分一致。在卵巢颗粒细胞中观察到中等水平的mRNA，在卵泡膜层和卵母细胞中的表达较低。在睾丸中，在Leydig和小管特异性生殖细胞中观察到β聚糖mRNA。这是第一份详细描述β聚糖在哺乳动物生殖组织中分布的综合报告。本研究结果说明并支持β聚糖在这些组织中的TGF β和/或TGF β受体抑制剂作用中的调节作用的假设。
Betaglycan was reported by our laboratory to serve as an inhibin binding protein and to facilitate the antagonism of activin signaling. Although an accessory receptor for TGFbeta and inhibin, its distribution within reproductive tissues remains largely unexplored. Histochemical analyses reveal betaglycan protein and mRNA distributed throughout the rat reproductive axis. In the brain, betaglycan mRNA is localized in discrete regions of the forebrain and brain stem, including olfactory, septal, and hypothalamic nuclei. In the pituitary, moderate levels of betaglycan protein and mRNA were observed in the anterior and intermediate lobes. Betaglycan immunoreactivity was colocalized with all the pituitary cell subtypes, to the greatest extent with the gonadotrope population. In the gonads, betaglycan mRNA was localized in cellular compartments, coinciding with its protein for the most part. Moderate levels of mRNA were observed in ovarian granulosa cells, with lower expression in the thecal layer and the oocyte. In the testes, betaglycan mRNA was observed in the Leydig and tubule-specific germ cells. This is the first comprehensive report detailing the distribution of betaglycan in mammalian reproductive tissues. The present findings illustrate and support the hypothesis of a modulatory role for betaglycan in TGFbeta and/or inhibin effects in these tissues.