Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
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DOI：
10.1128/mbio.00208-10
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发表时间：
2010-09-01
期刊：
影响因子：
6.4
通讯作者：
Lipkin, W. Ian
中科院分区：
文献类型：
--
作者：
Quan, Phenix-Lan;Firth, Cadhla;Lipkin, W. Ian
蝙蝠是新出现的人畜共患病毒的宿主，这些病毒包括溶血病毒、丝状病毒、副粘病毒和严重急性呼吸综合征冠状病毒（sars - cov），可对人类和动物健康产生深远影响。在一个关注在人与蝙蝠接触可能促进更有效的病毒种间传播的情况下发现病原体的项目中，我们调查了从人类经常光顾的尼日利亚洞穴中收集的蝙蝠的胃肠道组织。冠状病毒共识PCR和无偏倚高通量焦磷酸测序结果显示，在德国商业狐蝠（Hipposideros commersoni）中存在与sars冠状病毒相关的冠状病毒序列。额外的基因组测序表明，该病毒与包括SARS-CoV在内的2b亚群冠状病毒不同，它是独特的，在Mand N基因和两个保守的茎环II基序之间包含三个重叠的开放阅读框。结合这些特征进行的系统发育分析表明，该病毒代表了第2类冠状病毒中的一个新的亚群。蝙蝠（翼翅目、大翼翅亚目和小翼翅目）是多种病毒的宿主，可引起人类和牲畜疾病，包括导致2003年全球SARS爆发的严重急性呼吸综合征冠状病毒（SARS- cov）。由于野生动物监测的扩大以及病原体发现新工具的开发和应用，蝙蝠所携带病毒的多样性才刚刚开始被了解。本文描述了一种新的冠状病毒，一种具有独特基因组组织的冠状病毒，可能为冠状病毒的进化和生物学提供见解。
Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the Mand N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.IMPORTANCE Bats (order Chiroptera, suborders Megachiroptera and Microchiroptera) are reservoirs for a wide range of viruses that cause diseases in humans and livestock, including the severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for the global SARS outbreak in 2003. The diversity of viruses harbored by bats is only just beginning to be understood because of expanded wildlife surveillance and the development and application of new tools for pathogen discovery. This paper describes a new coronavirus, one with a distinctive genomic organization that may provide insights into coronavirus evolution and biology.