4D Printing of Multi‐Responsive Membrane for Accelerated In Vivo Bone Healing Via Remote Regulation of Stem Cell Fate
4D Printing of Multi‐Responsive Membrane for Accelerated In Vivo Bone Healing Via Remote Regulation of Stem Cell Fate
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4D 打印多响应膜，通过远程调节干细胞命运加速体内骨骼愈合
DOI：
10.1002/adfm.202103920
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发表时间：
2021
影响因子：
19
通讯作者：
Huiming Wang
中科院分区：
文献类型：
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作者：
Dongqi You;Guancong Chen;Chao Liu;Xin Ye;Shaolong Wang;Minyi Dong;Mouyuan Sun;Jianxiang He;Xiaowen Yu;Guanchen Ye;Qi Li;Junjie Wu;Jingjun Wu;Qian Zhao;Tao Xie;Mengfei Yu;Huiming Wang
基质微结构的动态调控是组织工程中控制干细胞命运的有效策略。在临床环境中将其转化为体内组织修复仍然具有挑战性，这需要对多尺度结构特征进行精确的时间控制。使用4D打印技术，制造由形状记忆聚合物（SMP）层和水凝胶层组成的多响应双层变形膜。SMP层具有响应性表面微结构，可以精确地在增殖和分化之间切换，从而促进骨形成。水凝胶层赋予膜数字调节其3D几何形状的能力，从而匹配临床场景中的特定宏观骨形状。作者的体内实验表明，与具有静态微结构的参考膜相比，4D变形膜在新骨形成方面表现出超过30%的改善。更重要的是，4D膜可以以非侵入性方式保形地包裹骨缺损模型，并且该策略可以扩展到涉及复杂组织缺损的修复。
Dynamic regulation of substrate micro‐structures is an effective strategy to control stem cell fate in tissue engineering. Translating this into in vivo tissue repair in a clinical setting remains challenging, which requires precise temporal control of multi‐scale structural features. Using 4D printing technique, a multi‐responsive bilayer morphing membrane consisting of a shape memory polymer (SMP) layer and a hydrogel layer, is fabricated. The SMP layer is featured with responsive surface micro‐structures, which can switch the phase between proliferation and differentiation precisely, thus promoting the bone formation. The hydrogel layer endows the membrane with the ability to digitally regulate its 3D geometry, matching the specific macroscopic bone shape in clinical scenario. The authors’ in vivo experiments show that the 4D shape‐shifting membrane exhibits over 30% improvement in new bone formation in comparison to a reference membrane with static micro‐structure. More importantly, the 4D membrane can conformally wrap a bone defect model in a non‐invasive way and this strategy can be extended to repairs involving complex tissue defects.