KINETICS OF RESEALING OF WASHED ERYTHROCYTE-GHOSTS
KINETICS OF RESEALING OF WASHED ERYTHROCYTE-GHOSTS
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DOI：
10.1007/bf01868173
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发表时间：
1975-01-01
影响因子：
2.4
通讯作者：
JOHNSON, RM
中科院分区：
文献类型：
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作者：
JOHNSON, RM
重新密封的动力学，定义为恢复不渗透性的大分子，洗好的人红细胞血影已被确定。在温度高于20-25°C的等渗盐中，再密封过程是一级反应，速率常数为0.01-0.15 min−1。在20°C以下，发生再密封，但观察到长的滞后期。红细胞膜的其他性质表明在约20°C时发生转变，并且重新密封速率可能是膜流动性的量度。还观察到温度引起的再密封鬼体积的减少。确定了离子强度对再密封参数的影响。低离子强度缓冲液防止再密封，这也与作为脂质相关事件的再密封一致。微管破坏药物的效果和变化的方法制备鬼也被描述。
The kinetics of resealing, defined as the recovery of impermeability to macromolecules, of well-washed human erythrocyte ghosts has been determined. The resealing process is first-order at temperatures above 20–25°C in isotonic salt, with rate constants ranging from 0.01–0.15 min−1. Below 20°C, resealing occurs, but a long lag period is observed. Other erythrocyte membrane properties suggest a transition at about 20°C, and it is possible that resealing rates are a measure of membrane fluidity. A temperature-induced reduction of resealed ghost volume was also observed. The effect of ionic strength on resealing parameters was determined. Low ionic strength buffers prevent resealing, which is also consistent with resealing as a lipid-related event. The effect of microtubule disrupting drugs and changes in the method of preparing ghosts are also described.