Induction and peak gene expression of insulin-like growth factor II follow that of myogenin during differentiation of BC3H-1 muscle cells.
Induction and peak gene expression of insulin-like growth factor II follow that of myogenin during differentiation of BC3H-1 muscle cells.
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在 BC3H-1 肌细胞分化过程中，胰岛素样生长因子 II 的诱导和基因表达峰值遵循肌细胞生成素的诱导和峰值基因表达。
DOI：
10.1016/s0006-291x(05)80301-8
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发表时间：
1992
影响因子：
3.1
通讯作者：
Rosenthal,SM
中科院分区：
文献类型：
--
作者：
Brown,EJ;Hsiao,D;Rosenthal,SM
胰岛素样生长因子 (IGF) 通过激素和/或自分泌/旁分泌机制发挥作用，刺激肌肉细胞的分化。先前的研究表明，IGF 刺激肌肉细胞分化的一种机制是增加肌细胞生成素的表达，肌细胞生成素是一种调节肌肉特异性基因表达的 DNA 结合蛋白。虽然外源性 IGF 肽可增加肌细胞生成素 mRNA，但内源性产生的 IGF 肽在肌细胞生成素表达中的作用尚未确定。此外，IGF 在调节 Id 表达中的潜在作用也是未知的，Id 是成肌细胞中的一种蛋白质，可以抑制肌生成素样肽的作用。在本研究中，我们研究了 BC3H-1 小鼠肌肉细胞分化过程中肌细胞生成素和 IGF-II mRNA 积累的动力学，并探讨了 IGF 在调节 Id 表达中的潜在作用。在血清撤除诱导的分化过程中，肌细胞生成素表达的诱导先于IGF-II（这些细胞表达的主要IGF肽）。此外，Id表达在无血清培养基中两小时内下降并且不受IGF处理的影响。因此，这些研究表明，在 Id 减少和肌细胞生成素基因表达诱导发生后，内源产生的 IGF-II 可能会刺激肌肉细胞分化。
Acting through hormonal and/or autocrine/paracrine mechanisms, the insulin-like growth factors (IGFs) stimulate the differentiation of muscle cells. Previous studies have suggested that one mechanism by which IGFs stimulate muscle cell differentiation is by increasing the expression of myogenin, a DNA binding protein that regulates the expression of muscle-specific genes. While exogenous IGF peptides increase myogenin mRNA, the role of endogenously produced IGF peptides in myogenin expression has not been established. In addition, the potential role of IGFs in regulating the expression of Id, a protein in myoblasts that can inhibit the action of myogenin-like peptides, is also unknown. In the present study, we have examined the kinetics of accumulation of myogenin and IGF-II mRNAs during differentiation of BC3H-1 mouse muscle cells and have explored the potential role of IGFs in regulating Id expression. During differentiation induced by serum withdrawal, induction of myogenin expression preceded that of IGF-II, the principal IGF peptide expressed by these cells. In addition, Id expression decreased within two hours in serum-free medium and was not affected by IGF treatment. Thus, these studies suggest that endogenously-produced IGF-II may stimulate muscle cell differentiation after both the decrease in Id and the induction of myogenin gene expression have occurred.