Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis.
Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis.
复制标题
焦亡期间监测钙通量和溶酶体胞吐作用。
DOI：
10.1007/978-1-0716-3040-2_14
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Bergsbaken,Tessa
中科院分区：
文献类型：
--
作者：
Loomis,WendyP;Bergsbaken,Tessa
炎性半胱天冬酶（半胱天冬酶-1、半胱天冬酶-4、半胱天冬酶-5、半胱天冬酶-11）的炎性体介导的活化启动导致促炎性细胞死亡或焦亡的细胞事件级联。gasdermin D的蛋白水解裂解导致形成允许释放成熟细胞因子IL-1β和IL-18的跨膜孔。Gasdermin孔还允许钙通过质膜流入，触发溶酶体区室与细胞表面的融合，并在称为溶酶体胞吐的过程中将其内容物释放到细胞外环境中。本章概述了炎症性半胱天冬酶激活后测量钙通量、溶酶体胞吐和膜破裂的方法。
Inflammasome-mediated activation of inflammatory caspases (caspase-1, caspase-4, caspase-5, caspase-11) initiates a cascade of cellular events that lead to proinflammatory cell death, or pyroptosis. Proteolytic cleavage of gasdermin D results in the formation of transmembrane pores that allow the release of mature cytokines IL-1β and IL-18. Gasdermin pores also allow calcium influx through the plasma membrane, triggering the fusion of lysosomal compartments with the cell surface and release of their contents into the extracellular milieu in a process termed lysosome exocytosis. This chapter outlines methods for measuring calcium flux, lysosome exocytosis, and membrane disruption after inflammatory caspase activation.