Cytological and pharmacological evidence that biotrophic fungi trigger different cell death execution processes in host and nonhost cells during the hypersensitive response
Cytological and pharmacological evidence that biotrophic fungi trigger different cell death execution processes in host and nonhost cells during the hypersensitive response
复制标题
DOI：
10.1016/s0885-5765(03)00088-2
复制
发表时间：
2003-05-01
影响因子：
2.7
通讯作者：
Heath, MC
中科院分区：
文献类型：
--
作者：
Christopher-Kozjan, R;Heath, MC
活表皮细胞经历一个过敏反应的渗透由锈病或白粉病真菌表现出两种不同的模式的细胞变化，发生从细胞质流停止的时间到原生质体崩溃。在非宿主细胞中，这一死亡过程在不到1小时内完成，trans-vacuolar链流停止后最初仍然可见，Brownian样运动被认为是在颗粒细胞质成分，和植物细胞核通常与细胞内真菌。在抗性宿主细胞中，跨空泡链消失的细胞质流停止，没有看到在细胞质中的运动，有没有一致的植物细胞核与真菌的协会，和死亡过程需要2小时或更长时间。没有一种类型的细胞死亡是模仿的应用CuCl 2，水杨酸，或KCN，也没有个别独特的形态特征的细胞死亡，他们引发的变化与化学浓度。药理学研究表明，宿主和非宿主细胞死亡需要细胞外钙离子的流入，蛋白激酶活性和蛋白质合成，但不是细胞外活性氧的产生。具有RGD基序的肽的应用仅在一种抗性宿主中延迟细胞死亡，而半胱天冬酶活性抑制剂仅在非宿主组合中延迟细胞死亡。这些和其他结果表明，一些锈病和白粉病特异性抗性基因在寄主品种可能控制不同的细胞解体过程中的过敏反应比在非寄主植物中触发。(C)2003 Elsevier Ltd.保留所有权利。
Living epidermal cells undergoing a hypersensitive response to penetration by a rust or a powdery mildew fungus exhibited two distinct patterns of cellular changes that occurred from the time of the cessation of cytoplasmic streaming to protoplast collapse. In nonhost cells, this death process was completed in less than I h, trans-vacuolar strands initially remained visible after streaming stopped, Brownian-like motion was seen in particulate cytoplasmic components, and the plant nucleus was usually associated with the intracellular fungus. In resistant host cells, trans-vacuolar strands disappeared as cytoplasmic streaming stopped, no motion was seen in the cytoplasm, there was no consistent association of the plant nucleus with the fungus, and the death process took 2 h or more. Neither type of cell death was mimicked by the application of CuCl2, salicylic acid, or KCN, nor did the individually distinctive morphological features of the cell deaths that they triggered change with chemical concentration. Pharmacological studies suggested that host and nonhost cell death required an influx of extracellular calcium, protein kinase activity and protein synthesis, but not the generation of extracellular reactive oxygen species. The application of peptides with an RGD motif delayed cell death in only one resistant host, and inhibitors of caspase activity delayed cell death only in the nonhost combinations. These and other results suggest that some rust- and powdery mildew-specific resistance genes in host cultivars may control a different cell dismantling process during the hypersensitive response than that triggered in nonhost plants. (C) 2003 Elsevier Ltd. All rights reserved.