Sensing Picomolar Concentrations of RNA Using Switchable Plasmonic Chirality
Sensing Picomolar Concentrations of RNA Using Switchable Plasmonic Chirality
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DOI：
10.1002/anie.201807029
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发表时间：
2018-10-08
影响因子：
16.6
通讯作者：
Liedl, Tim
中科院分区：
文献类型：
--
作者：
Funck, Timon;Nicoli, Francesca;Liedl, Tim
检测生物样品中的小序列RNA，如microRNA或病毒RNA，需要高度敏感和特异性的方法。在这里，已经使用了可重构的DNA折纸模板，其中结构上的金纳米棒的手性排列可以导致产生强圆二色性（CD）。交叉样DNA结构的转换是通过添加核酸序列来实现的，核酸序列通过特异性分子识别将结构捕获在一种可能的手性状态中。因此，可以通过等离子体CD光谱中产生的变化来检测特定序列。我们展示了从丙型肝炎病毒基因组中灵敏和选择性地检测靶RNA序列。RNA与作为锁定机制的一部分的互补序列结合，这导致形成具有不同光学响应的等离子体系统的限定状态。通过这种方法，我们能够在低至100 μ m的浓度下检测到这种特定的RNA序列。
Detecting small sequences of RNA in biological samples such as microRNA or viral RNA demands highly sensitive and specific methods. Here, a reconfigurable DNA origami template has been used where a chiral arrangement of gold nanorods on the structure can lead to the generation of strong circular dichroism (CD). Switching of the cross-like DNA structure is achieved by the addition of nucleic acid sequences, which arrests the structure in one of the possible chiral states by specific molecular recognition. A specific sequence can thus be detected through the resulting changes in the plasmonic CD spectrum. We show the sensitive and selective detection of a target RNA sequence from the hepatitis C virus genome. The RNA binds to a complementary sequence that is part of the lock mechanism, which leads to the formation of a defined state of the plasmonic system with a distinct optical response. With this approach, we were able to detect this specific RNA sequence at concentrations as low as 100 pm.