Killing of spores of Bacillus species by cetyltrimethylammonium bromide
Killing of spores of Bacillus species by cetyltrimethylammonium bromide
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十六烷基三甲基溴化铵杀死芽孢杆菌属的孢子
DOI：
10.1111/jam.14242
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发表时间：
2019-05-01
影响因子：
4
通讯作者：
Setlow, P.
中科院分区：
文献类型：
--
作者：
Dong, W.;Green, J.;Setlow, P.
目的研究阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）对芽孢杆菌芽孢的杀灭效果。方法和结果研究了CTAB触发芽孢杆菌孢子的大贮库的吡啶二羧酸（DPA）与Ca 2+（CaDPA）的1：1螯合物（CaDPA）的释放和杀灭芽孢的能力。CTAB引发的CaDPA从枯草芽孢杆菌、蜡状芽孢杆菌和巨大芽孢杆菌的孢子中释放，但随后没有完成萌发。在较高的温度下，由CTAB引发的CaDPA释放增加，并且对于B是最佳的。枯草芽孢杆菌孢子，pH9，中心点4和30 μ g ml（-1）CTAB。CTAB也杀死芽孢杆菌孢子，如平板计数和处理的休眠孢子的活体染色所示，以及在它们萌发后。然而，B。cereus和B.巨大芽孢对CTAB的敏感性高于B。枯草芽孢CTAB处理的同基因B孢子的CaDPA释放和杀灭。还检测了缺乏萌发蛋白的枯草杆菌突变体，并与公知的萌发剂十二胺对孢子的作用进行了比较，以确定CTAB如何对孢子发挥其作用。结论本研究的结果表明，CTAB杀死三种芽孢杆菌的孢子，可能是通过破坏孢子内膜，虽然它也可能是由该试剂的一些杀死后，其触发孢子萌发。研究的意义和影响这项工作的结果表明，CTAB也是一种消毒剂，但也是一种杀孢子剂，并可能是一个有用的辅助孢子去污，特别是在较高的温度。
Aims To investigate effects of the cationic surfactant cetyltrimethylammonium bromide (CTAB), a disinfectant, on spores of Bacillus species. Methods and Results The ability of CTAB to trigger release of Bacillus spores' large depot of dipicolinic acid (DPA) in a 1 : 1 chelate with Ca2+ (CaDPA), and to kill spores was investigated. CTAB-triggered CaDPA release from spores of Bacillus subtilis, Bacillus cereus and Bacillus megaterium, but was not followed by completion of germination. CaDPA release triggered by CTAB increased at higher temperatures, and was optimal for B. subtilis spores at pH 9 center dot 4 and 30 mu g ml(-1) CTAB. CTAB also killed Bacillus spores as shown by plate counts and vital staining of treated dormant spores, and after their germination. However, B. cereus and B. megaterium spores were more CTAB-sensitive than were B. subtilis spores. CaDPA release from and killing of CTAB-treated spores of isogenic B. subtilis mutants lacking germination proteins was also examined, and compared with effects of the well-known germinant dodecylamine on spores, to determine how CTAB exerts its effects on spores. Conclusions The results of this investigation showed that CTAB kills spores of three Bacillus species, perhaps by damaging the spore inner membrane, although it is also possible that some killing by this agent follows its triggering of spore germination. Significance and Impact of the Study The results of this work indicate that CTAB is also a disinfectant, but also a sporicide, and may be a useful adjunct in spore decontamination, especially at higher temperatures.