Evolution of the acyl-CoA binding protein (ACBP)
Evolution of the acyl-CoA binding protein (ACBP)
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DOI：
10.1042/bj20050664
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发表时间：
2005-12-01
影响因子：
4.1
通讯作者：
Knudsen, J
中科院分区：
文献类型：
--
作者：
Burton, M;Rose, TM;Knudsen, J
酰基辅酶A结合蛋白（ACBP）是一种10 kDa的蛋白质，以高亲和力结合C-12-C-22酰基辅酶A酯。体外和体内实验表明，它参与多种细胞任务，包括脂肪酸生物合成的调节，酶的调节，细胞内酰基辅酶A池大小的调节，酰基辅酶A酯的β-氧化，囊泡运输，复合脂质合成和基因调控的捐赠。本研究通过对ACBP进化历史的描述，全面了解其在物种间的进化和分布。ACBP同源物在所有四个真核生物界，动物界，植物界，真菌和原生生物，和11个真细菌物种中被鉴定。ACBP同源物没有检测到，我的其他已知的细菌物种，或在古细菌。几乎所有含ACBP的细菌都对植物或动物具有致病性，这表明所有的ACBP基因都可以通过水平基因转移从真核宿主中获得。许多细菌、真菌和高等真核生物物种只含有单一的ACBP同源物。然而，一些物种，从原生动物到脊椎动物，已经通过基因复制和/或逆转录酶事件进化出两到六个谱系特异性旁系同源物。ACBP蛋白在门中高度保守，并且大多数ACBP基因经受强纯化选择。实验证据表明ACBP的功能从酵母到人类一直是保守的，并且多个谱系特异性旁系同源物已经进化改变了功能。ACBP在进化早期的出现表明ACBP在酰基辅酶A代谢（包括神经酰胺合成和信号传导）中的基本作用。
Acyl-CoA-binding protein (ACBP) is a 10kDa protein that binds C-12-C-22 acyl-CoA esters with high affinity. In vitro and in vivo experiments suggest that it is involved in multiple cellular-tasks including modulation of fatty acid biosynthesis, enzyme regulation, regulation of the intracellular acyl-CoA pool size, donation of acyl-CoA esters for beta-oxidation, vesicular trafficking, complex lipid synthesis and gene regulation. In the present study, we delineate the evolutionary history of ACBP to get it complete Picture of its evolution and distribution among species. ACBP homologues were identified in all four eukaryotic kingdoms, Animalia, Plantae, Fungi and Protista, and eleven eubacterial species. ACBP homologues were not detected in,my other known bacterial species, or in archaea. Nearly all of the ACBP-containing bacteria are pathogenic to plants or animals, suggesting that all ACBP gene could have been acquired from a eukaryotic host by horizontal gene transfer. Many bacterial, fungal and higher eukaryotic species only harbour it single ACBP homologue. However, a number of species, ranging from protozoa to vertebrates, have evolved two to six lineage-specific paralogues through gene duplication and/or retrotransposit ion events. The ACBP protein is highly conserved across phylums, and the majority of ACBP genes are subjected to strong purifying selection. Experimental evidence indicates that the function of ACBP has been conserved from yeast to humans and that the multiple lineage-specific paralogues have evolved altered functions. The appearance of ACBP very early on in evolution points towards it fundamental role of ACBP in acyl-CoA metabolism, including ceramide synthesis and in signalling.