COMPARTMENTALIZATION OF THE PERIPLASMIC SPACE AT DIVISION SITES IN GRAM-NEGATIVE BACTERIA
COMPARTMENTALIZATION OF THE PERIPLASMIC SPACE AT DIVISION SITES IN GRAM-NEGATIVE BACTERIA
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DOI：
10.1128/jb.168.3.1430-1438.1986
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发表时间：
1986-12-01
影响因子：
3.2
通讯作者：
ROTHFIELD, LI
中科院分区：
文献类型：
--
作者：
COOK, WR;MACALISTER, TJ;ROTHFIELD, LI
相差和连续切片电子显微镜被用来研究局部质粒的模式，发生时，鼠伤寒沙门氏菌和大肠杆菌细胞暴露于高渗溶液的蔗糖。在分裂的细胞中，新生的隔膜两侧是隔膜周质团的局部区域。在随机生长的人群中，与隔向内生长无关的plasmicrobay聚集在细胞的中点，并在1/4和3/4细胞长度。局部区域的plasma细胞被连续的粘附区所限制，类似于先前在S. lkyD突变体中描述的间隔周围环形粘附区。鼠伤寒杆菌（T. J.马卡利斯特，B. MacDonald和L. I. Rothfield，Proc. Natl. Acad. Sci. USA 80：1372-1376，1983）。当细胞分裂被生长的divC（Ts）细胞在升高的温度下阻断时，质膜的局部区域沿沿着无隔丝聚集在对应于当细胞分裂被允许恢复到允许温度时发生隔膜形成的位置。两者合计的结果是一致的模型，其中扩展区的粘附定义本地化的隔室内的周质空间，主要位于未来的网站的细胞分裂。
Phase-contrast and serial section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmolysis. In randomly growing populations, plasmolysis bays that were not associated with septal ingrowth were clustered at the midpoint of the cell and at 1/4 and 3/4 cell lengths. The localized regions of plasmolysis were limited by continuous zones of adhesion that resembled the periseptal annular adhesion zones described previously in lkyD mutants of S. typhimurium (T. J. MacAlister, B. MacDonald, and L. I. Rothfield, Proc. Natl. Acad. Sci. USA 80:1372-1376, 1983). When cell division was blocked by growing divC(Ts) cells at elevated temperatures, the localized regions of plasmolysis were clustered along the aseptate filaments at positions that corresponded to sites where septum formation occurred when cell division was permitted to resume by a shift back to the permissive temperature. Taken together the results are consistent with a model in which extended zones of adhesion define localized compartments within the periplasmic space, predominantly located at future sites of cell division.