Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China
Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China
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DOI：
10.4238/2015.september.21.16
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Chen, Z. W.
中科院分区：
文献类型：
--
作者：
Du, X. Y.;Li, W.;Chen, Z. W.
虽然沙土鼠多年来已被广泛应用于生物学研究的许多领域，但目前还没有有效的遗传质量控制系统可用。在本研究中，我们试图建立一个用于质量控制的微卫星标记体系，并对两个实验室沙鼠群体和一个野生沙鼠群体的137个微卫星座位进行了优化分析。对平均有效等位基因数、Shannon‘s信息指数平均值和平均HE值进行独立样本t检验，结果表明137个微卫星标记中有28个对沙土鼠遗传控制具有信息性。对4个实验沙鼠群体和1个野生沙鼠群体的28个微卫星座位分析表明，广州沙鼠的等位基因数为1.9639，西北野生沙鼠的等位基因数为6.6071。北京、西北、杭州、大连和广州人群的HO/HE平均值分别为0.6236/0.3802、0.6671/0.4159、0.4185/0.3464、0.4592/0.3821和0.3972/0.4167。广州群体分化程度最大，具有较高的R-ST和Nei‘s标准遗传距离。方差分析显示5个群体间存在较高的遗传分化(F-ST=0.296)。建立的微卫星系统是有效的，将对未来沙土鼠品种的遗传质量控制和监测研究具有重要意义。
Although gerbils have been widely used in many areas of biological research over many years, there is currently no effective genetic quality control system available. In the present study, we sought to establish a microsatellite marker system for quality control and conducted an optimized analysis of 137 microsatellite loci in two laboratory gerbil populations and one wild population. Independent sample t-tests on the mean effective allele number, mean of Shannon's information index, and mean HE suggested that 28 of the 137 microsatellite markers were informative for gerbil genetic control. Analysis of 4 laboratory gerbil populations and 1 wild population using the 28 microsatellite loci indicated that allele numbers varied from 1.9639 (Guangzhou, GZ) to 6.6071 (North-West wild, NW). The average of HO versus HE was 0.6236/0.3802, 0.6671/0.4159, 0.4185/0.3464, 0.4592/0.3821, and 0.3972/0.4167 for the Beijing, NW, Hangzhou, Dalian, and GZ populations, respectively. The GZ population showed the greatest differentiation, having higher R-ST and Nei's standard genetic distances. An AMOVA revealed high genetic differentiation among the five populations (F-ST = 0.296). The microsatellite system established here is effective and will be important in future studies for genetic quality control and monitoring of gerbil breeds.