Use of Nerve Conduction Velocity to Assess Peripheral Nerve Health in Aging Mice
Use of Nerve Conduction Velocity to Assess Peripheral Nerve Health in Aging Mice
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DOI：
10.1093/gerona/glu208
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发表时间：
2015-11-01
影响因子：
5.1
通讯作者：
Van Remmen, Holly
中科院分区：
文献类型：
--
作者：
Walsh, Michael E.;Sloane, Lauren B.;Van Remmen, Holly
神经传导速度（NCV），即电信号沿周围神经传播的速度，在临床上用于评估人类的神经功能。周围神经功能的下降与许多与年龄相关的病症有关。虽然多项研究表明人类 NCV 随着年龄的增长而下降，但关于年龄对小鼠周围神经 NCV 影响的信息很少。在这项研究中，我们评估了 4 至 32 个月龄的雄性和雌性 C57Bl/6 小鼠的 NCV。我们观察到 20 月龄后雄性和雌性小鼠的 NCV 均有所下降。感觉 NCV 以及运动神经衰老过程中的下降率均存在性别差异；与雄性小鼠相比，雌性小鼠的感觉 NCV 较慢，运动神经与年龄相关的衰退也较慢。我们还在 30 个月大的雌性小鼠中测试了饮食限制对 NCV 的影响。饮食限制可以防止坐骨 NCV 与年龄相关的下降，但不能防止其他神经。由于 NCV 在临床上与神经功能的评估相关，因此我们建议在评估可延长小鼠寿命的遗传和药物干预措施时使用 NCV 来评估健康寿命。
Nerve conduction velocity (NCV), the speed at which electrical signals propagate along peripheral nerves, is used in the clinic to evaluate nerve function in humans. A decline in peripheral nerve function is associated with a number of age-related pathologies. While several studies have shown that NCV declines with age in humans, there is little information on the effect of age on NCV in peripheral nerves in mice. In this study, we evaluated NCV in male and female C57Bl/6 mice ranging from 4 to 32 months of age. We observed a decline in NCV in both male and female mice after 20 months of age. Sex differences were detected in sensory NCV as well as the rate of decline during aging in motor nerves; female mice had slower sensory NCV and a slower age-related decline in motor nerves compared with male mice. We also tested the effect of dietary restriction on NCV in 30-month-old female mice. Dietary restriction prevented the age-related decline in sciatic NCV but not other nerves. Because NCV is clinically relevant to the assessment of nerve function, we recommend that NCV be used to evaluate healthspan in assessing genetic and pharmacological interventions that increase the life span of mice.