Proton-pumping F-ATPase plays an important role in Streptococcus mutans under acidic conditions
Proton-pumping F-ATPase plays an important role in Streptococcus mutans under acidic conditions
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DOI：
10.1016/j.abb.2019.03.014
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发表时间：
2019-05-15
影响因子：
3.9
通讯作者：
Nakanishi-Matsui, Mayumi
中科院分区：
文献类型：
--
作者：
Sekiya, Mizuki;Izumisawa, Shintaro;Nakanishi-Matsui, Mayumi
变形链球菌是一种主要寄生在牙齿表面的细菌，是龋齿的主要病原体。细菌代谢糖产生酸，导致牙菌斑中的酸性微环境。因此，变形链球菌应该具有在酸性条件下存活的机制。在本研究中，我们报道了埃希氏菌线圈质子泵送f型atp酶（f - atp酶）抑制剂对变形链球菌酶活性的影响，以及在酸性条件下变形链球菌的生长和存活。皮杉酚、姜黄素和去甲氧基姜黄素能显著降低变形链球菌f - atp酶的活性。有趣的是，这些化合物在pH为5.3时抑制变形链球菌的生长，而在pH为7.3时则没有。它们也显著降低了变形链球菌在pH 4.3孵育后的集落形成能力，而在pH 7.3孵育时基本没有影响。这些观察结果表明，变形链球菌在酸性条件下对f - atp酶抑制剂高度敏感，f - atp酶在这种细菌的耐酸性中起重要作用。
Streptococcus mutans, a bacterium mainly inhabiting the tooth surface, is a major pathogen of dental caries. The bacterium metabolizes sugars to produce acids, resulting in an acidic microenvironment in the dental plaque. Hence, S. mutans should possess a mechanism for surviving under acidic conditions. In the current study, we report the effects of inhibitors of Escherichia coil proton-pumping F-type ATPase (F-ATPase) on the activity of S. mutans enzyme, and the growth and survival of S. mutans under acidic conditions. Piceatannol, curcumin, and demethoxycurcumin strongly reduced the ATPase activity of S. mutans F-ATPase. Interestingly, these compounds inhibited the growth of S. mutans at pH 5.3 but not at pH 7.3. They also significantly reduced the colony-forming ability of S. mutans after incubation at pH 4.3, while showing essentially no effect at pH 7.3. These observations indicate that S. mutans is highly sensitive to F-ATPase inhibitors under acidic conditions and that F-ATPase plays an important role in acid tolerance of this bacterium.