Ethylene biosynthesis and expression of related genes in loquat fruit at different developmental and ripening stages
Ethylene biosynthesis and expression of related genes in loquat fruit at different developmental and ripening stages
复制标题
不同发育成熟阶段枇杷果实乙烯生物合成及相关基因表达
DOI：
10.1016/j.scienta.2011.07.019
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发表时间：
2011-09-14
影响因子：
4.3
通讯作者：
Chen, Kun-Song
中科院分区：
文献类型：
--
作者：
Jiang, Tian-Mei;Wang, Ping;Chen, Kun-Song
从非跃变型枇杷果实中克隆了3个乙烯生物合成相关基因EjACS 1、EjACO 1和EjACO 2。CV.洛阳青）。实时定量PCR（Q-PCR）分析表明，EjACS 1和EjACO 1基因在果实中特异表达，而EjACO 2基因在叶片和花瓣中也有表达。EjACO 2基因在果实发育过程中的表达模式与乙烯的产生一致，在果实转色时达到高峰。EjACS 1、EjAC 01和EjACO 2在采后成熟枇杷的整个20 ℃贮藏期间均表现出低转录水平。这是首次对枇杷果实中乙烯生物合成相关基因的表达进行研究。枇杷果实发育过程中乙烯释放量和呼吸速率均呈跃变型增加，EjACO 2可能在此过程中起重要作用。(C)2011爱思唯尔有限公司版权所有。
Three ethylene biosynthesis related genes, EjACS1, EjACO1, and EjACO2, were cloned from the non-climacteric loquat fruit (Eriobotrya japonica Lindl. cv. Luoyangqing). Real-time quantitative PCR (Q-PCR) analysis showed the specific expression of the EjACS1 and EjACO1 genes in fruit, whereas EjACO2 was also expressed in leaves and petals. The expression pattern of EjACO2 was consistent with ethylene production during fruit development, which reached a peak when the fruit color was turning. EjACS1, EjAC01, and EjACO2 all showed low transcript levels throughout 20 degrees C storage in the postharvest ripening loquat. This is the first time that the expression of ethylene biosynthesis related genes has been studied in loquat fruit. Climacteric increases in ethylene production and respiration rate were observed during the development of loquat fruit, and EjACO2 may play an important role in this process. (C) 2011 Elsevier B.V. All rights reserved.