The probiotic activity of Lactobacillus murinus against food allergy
The probiotic activity of Lactobacillus murinus against food allergy
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DOI：
10.1016/j.jff.2016.06.006
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发表时间：
2016-08-01
影响因子：
5.6
通讯作者：
Jan, Tong-Rong
中科院分区：
文献类型：
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作者：
Huang, Chung-Hsiung;Shen, Chien-Chang;Jan, Tong-Rong
研究了鼠乳杆菌对抗食物过敏的益生菌活性。口服 L. murinus 可恢复食物过敏小鼠肠道菌群恶化的情况，并减弱过敏反应，包括过敏原诱导的腹泻、肥大细胞激活和血清 IgE 产生。通过施用鼠乳杆菌，脾细胞产生 IFN-γ 和 IL-4 分别得到增强和抑制。一致地，在十二指肠中观察到IL-4和GATA3的表达降低以及IFN-γ和T-bet的表达增加。此外，L. murinus 还能增强肠道 CD11c(+) 细胞 IL-12 的产生并抑制 OX40 配体的表达。直接暴露于热灭活的鼠乳杆菌可增强肠系膜淋巴结细胞的 IFN-γ 分泌。这些发现表明，L. murinus 通过调节肠道菌群、肠道 CD11c(+) 细胞功能和 Th1/Th2 免疫平衡而具有抗过敏活性，并表明 L. murinus 可用作抗食物过敏的功能性益生菌。 (C) 2016 Elsevier Ltd. 保留所有权利。
The probiotic activity of Lactobacillus murinus against food allergy was investigated. Oral administration with L. murinus restored the deteriorated profile of enteric flora in mice with food allergy and attenuated allergic responses, including allergen-induced diarrhoea, mast cell activation, and serum IgE production. The production of IFN-gamma and IL-4 by splenocytes was enhanced and suppressed, respectively, by L. murinus administration. Concordantly, a decreased expression of IL-4 and GATA3 and an increased expression of IFN-gamma and T-bet were observed in the duodenum. Moreover, L. murinus enhanced IL-12 production and suppressed OX40 ligand expression by intestinal CD11c(+) cells. Direct exposure to heat-killed L. murinus enhanced IFN-gamma secretion by mesenteric lymph node cells. These findings demonstrate that L. murinus possesses anti-allergic activities via modulating enteric flora, intestinal CD11c(+) cell functionality and the Th1/Th2 immunobalance, and suggest that L. murinus may be employed as a functional probiotic against food allergy. (C) 2016 Elsevier Ltd. All rights reserved.