1-alpha,25-dihydroxyvitamin D-3 potentiates avian osteoclast activation by increasing the formation of zipper-like structure via Src/Rac1 signaling
1-alpha,25-dihydroxyvitamin D-3 potentiates avian osteoclast activation by increasing the formation of zipper-like structure via Src/Rac1 signaling
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1-α,25-二羟基维生素 D-3 通过 Src/Rac1 信号传导增加拉链样结构的形成，从而增强禽类破骨细胞的激活
DOI：
10.1016/j.bbrc.2018.05.048
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发表时间：
2018
影响因子：
3.1
通讯作者：
Liu Zong-Ping
中科院分区：
文献类型：
--
作者：
Wang Dong;Gu Jian-Hong;Feng Ling-Ling;Tong Xi-Shuai;Song Rui-Long;Zhao Hong-Yan;Bian Jian-Chun;Liu Xue-Zhong;Yuan Yan;Liu Zong-Ping
鸡骨代谢疾病影响鸡的生长发育和生产，其中许多疾病可以通过均衡营养和激素药物进行预防和控制。类固醇激素1α，25-二羟维生素D3在维持鸟类骨代谢平衡中起着关键作用。临床上1α，25-（OH）_2D_3已被用于治疗多种骨疾病。虽然已有研究表明1α，25-（OH）2D 3对破骨细胞的作用，但其作用机制尚不清楚。本研究利用分子和细胞生物学方法证实了1α，25-（OH）2D 3能促进鸡骨形成和活性，并上调骨吸收相关基因。此外，1α，25-（OH）2D 3还通过Src/Rac 1信号通路促进OC前体细胞拉链样结构的形成，增强OC活性，从而调节OC细胞骨架。这些发现为1α，25-（OH）2D 3在OC活性中的作用提供了新的认识。
Avian bone metabolism diseases affect the development and production of chickens, and many of these diseases can be prevented and controlled by balanced nutrition and hormone medicine. The steroid hormone 1α,25-dihydroxyvitamin D3plays a key role in maintaining the balance of avian bone metabolism. Clinically, 1α,25-(OH)2D3has been used to treat several bone diseases. Although several previous studies have investigated the effects of 1α,25-(OH)2D3on osteoclastogenesis, the mechanisms underpinning osteoclast (OC) activity remain largely unknown. Herein, we used molecular and cell biology approaches to demonstrate that 1α,25-(OH)2D3increases avian OC formation and activity, and upregulates bone resorption-related genes. Moreover, 1α,25-(OH)2D3regulates the OC cytoskeleton by increasing the formation of zipper-like structure in OC precursor cells to potentiate OC activity via the Src/Rac1 signaling pathway. These findings provide new insight into the role of 1α,25-(OH)2D3in OC activity.