Fow2, a Zn(II)2Cys6-type transcription regulator, controls plant infection of the vascular wilt fungus Fusarium oxysporum
Fow2, a Zn(II)2Cys6-type transcription regulator, controls plant infection of the vascular wilt fungus Fusarium oxysporum
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DOI：
10.1111/j.1365-2958.2006.05554.x
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Tsuge, Takashi
中科院分区：
文献类型：
--
作者：
Imazaki, Iori;Kurahashi, Makoto;Tsuge, Takashi
丝状真菌尖孢镰刀菌是一种土传寄生虫，其通过直接穿透根部并定殖维管组织而在多种作物中引起维管枯萎。在前期工作中，我们从甜瓜枯萎病菌F. oxysporum F.通过限制性内切酶介导的整合（REMI）诱变，B137的分子表征表明，该突变体在一个基因中具有单拷贝质粒插入，命名为FOW 2，其编码属于Zn（II）2Cys 6家族的推定的转录调节因子。REMI突变体B137和其他FOW 2靶向突变体完全丧失致病性，但在培养物中的营养生长和分生孢子形成没有受损。对绿色荧光蛋白（GFP）标记的野生型和突变株的侵染行为进行显微镜观察，发现突变株在侵入根系和定殖植物组织的能力上存在缺陷。FOW 2在F.感染不同植物的尖孢菌病原体。番茄枯萎病菌F. oxysporum F.番茄斑点病菌也失去了致病性。使用表达Fow 2-GFP和GFP-Fow 2融合蛋白的菌株验证Fow 2的核定位。这些数据有力地表明，FOW 2编码一个转录调节因子，控制F.尖孢菌属病原体
The filamentous fungus Fusarium oxysporum is a soil-borne parasite that causes vascular wilts in a wide variety of crops by directly penetrating roots and colonizing the vascular tissue. In previous work, we generated the non-pathogenic mutant B137 of the melon wilt pathogen F. oxysporum f. sp. melonis by using restriction enzyme-mediated integration (REMI) mutagenesis. Molecular characterization of B137 revealed that this mutant has a single-copy plasmid insertion in a gene, designated FOW2, which encodes a putative transcription regulator belonging to the Zn(II)2Cys6 family. The REMI mutant B137 and other FOW2-targeted mutants completely lost pathogenicity, but were not impaired in vegetative growth and conidiation in cultures. Microscopic observation of infection behaviours of green fluorescent protein (GFP)-marked wild-type and mutant strains revealed that the mutants were defective in their abilities to invade roots and colonize plant tissues. FOW2 is conserved in F. oxysporum pathogens that infect different plants. The FOW2-targeted mutants of the tomato wilt pathogen F. oxysporum f. sp. lycopersici also lost pathogenicity. Nuclear localization of Fow2 was verified using strains expressing Fow2-GFP and GFP-Fow2 fusion proteins. These data strongly suggest that FOW2 encodes a transcription regulator controlling the plant infection capability of F. oxysporum pathogens.