Stipe cell wall architecture varies with the stipe elongation of the mushroom Coprinopsis cinerea
Stipe cell wall architecture varies with the stipe elongation of the mushroom Coprinopsis cinerea
复制标题
灰鬼伞菌柄细胞壁结构随菌柄伸长而变化
DOI：
10.1016/j.funbio.2015.07.008
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发表时间：
2015-10-01
期刊：
影响因子：
2.5
通讯作者：
Yuan, Sheng
中科院分区：
文献类型：
--
作者：
Niu, Xin;Liu, Zhonghua;Yuan, Sheng
在伸长和非伸长的菌柄区域，细胞外壁表面都覆盖着大量的颗粒状突起，而在非伸长的菌柄区域，大量的颗粒状突起覆盖在细胞内壁表面。在非伸长的菌柄区域，探索了使用覆盖在细胞内壁和外壁表面的碱性无定形材料去除颗粒状突起。β-1，3-葡聚糖酶处理不仅去除了壁面上那些颗粒状突起和下面的无定形物质，而且还去除了大多数菌柄区域细胞壁上嵌入甲壳素微纤维的壁基质，但非伸长菌柄区域的细胞外壁表面保留了大部分壁基质。在顶端伸长区，几丁质微纤维在细胞内外壁上紧密横向排列，而在非伸长顶点区，细胞内壁上几丁质微纤维横向排列疏松，在细胞外壁上变得稀疏，甚至随机排列。我们认为，颗粒突起和无定形物质的表面沉积以及微纤维构筑和壁基质的改变可能导致壁塑性的丧失和柄伸长的停止。(C)2015年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
A large amount of granular protrusions overlie the outer cell wall surfaces in both elongating and non-elongating stipe regions but overlie the inner cell wall surfaces only in nonelongating stipe regions. Removal of granular protrusions using alkali, amorphous materials overlying on both the inner and outer cell wall surfaces were explored in the nonelongating stipe regions. beta-1,3-Glucanase treatment not only removed above those granular protrusions and underlying amorphous materials on the wall surfaces but also removed wall matrices embedding chitin microfibrils on the cell walls of most stipe regions, except for the outer cell wall surfaces of the non-elongating stipe regions where most of the wall matrices remained. The chitin microfibrils were closely and transversely arranged on both the inner and outer cell wall surfaces in the elongating apical stipe region, whereas they were loosely and transversely arranged on the inner cell wall surfaces and further became sparser and even randomly arranged on the outer cell wall surface in the non-elongating stipe regions. We propose that the surface deposition of granular protrusions and amorphous materials and the change of microfibril architecture and wall matrices may cause loss of wall plasticity and cessation of stipe elongation. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.