Extracellular ATP signaling is mediated by H₂O₂ and cytosolic Ca²⁺ in the salt response of Populus euphratica cells.
Extracellular ATP signaling is mediated by H₂O₂ and cytosolic Ca²⁺ in the salt response of Populus euphratica cells.
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DOI：
10.1371/journal.pone.0053136
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Sun J;Zhang X;Deng S;Zhang C;Wang M;Ding M;Zhao R;Shen X;Zhou X;Lu C;Chen S
细胞外ATP(EATP)参与调节植物生长和抗氧化防御；然而，eATP是否可能调节耐盐性在很大程度上还不清楚。我们利用共聚焦显微镜、非侵入性的振动离子选择微电极和实时定量聚合酶链式反应分析来评价eATP在耐盐木本植物胡杨细胞培养物耐盐中的生理意义。应用氯化钠(200 MM)刺激可引起[eATP]一过性升高。我们用苏拉明和PPADS阻断P2受体，并应用己糖激酶-葡萄糖的ATP捕获系统来研究eATP的作用。我们发现eATP调控盐适应所需的广泛的细胞过程，包括液泡Na+区隔、跨质膜Na+/H+交换、K+稳态、活性氧物种调节以及与K+/Na+稳态和质膜修复相关基因的盐响应表达。此外，我们还发现，在胡杨细胞中，eATP信号是由H_2O_2和细胞内高盐反应释放的钙离子所介导的。我们的结论是，盐诱导的eATP是由PM中的嘌呤受体感应的，这导致了下游信号的诱导，如H_2O_2和胞浆Ca~(2+)，这是上调与K+/Na+稳态和PM修复相关的基因所必需的。因此，在长时间的盐胁迫下，胡杨细胞的活力得以维持。
Extracellular ATP (eATP) has been implicated in mediating plant growth and antioxidant defense; however, it is largely unknown whether eATP might mediate salinity tolerance. We used confocal microscopy, a non-invasive vibrating ion-selective microelectrode, and quantitative real time PCR analysis to evaluate the physiological significance of eATP in the salt resistance of cell cultures derived from a salt-tolerant woody species, Populus euphratica. Application of NaCl (200 mM) shock induced a transient elevation in [eATP]. We investigated the effects of eATP by blocking P2 receptors with suramin and PPADS and applying an ATP trap system of hexokinase-glucose. We found that eATP regulated a wide range of cellular processes required for salt adaptation, including vacuolar Na+ compartmentation, Na+/H+ exchange across the plasma membrane (PM), K+ homeostasis, reactive oxygen species regulation, and salt-responsive expression of genes related to K+/Na+ homeostasis and PM repair. Furthermore, we found that the eATP signaling was mediated by H2O2 and cytosolic Ca2+ released in response to high salt in P. euphratica cells. We concluded that salt-induced eATP was sensed by purinoceptors in the PM, and this led to the induction of downstream signals, like H2O2 and cytosolic Ca2+, which are required for the up-regulation of genes linked to K+/Na+ homeostasis and PM repair. Consequently, the viability of P. euphratica cells was maintained during a prolonged period of salt stress.
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