Altered expression of Olr59, Ethe1, and Slc10a2 genes in the liver of F344 rats by neonatal thyroid hormone disruption.
Altered expression of Olr59, Ethe1, and Slc10a2 genes in the liver of F344 rats by neonatal thyroid hormone disruption.
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新生甲状腺激素破坏改变了 F344 大鼠肝脏中 Olr59、Ethe1 和 Slc10a2 基因的表达。
DOI：
10.1002/jat.3452
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
Matsubara K et al.
中科院分区：
文献类型：
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作者：
増田茜;増田雅美;中島宏之;小島弘幸;北村繁幸;浦丸直人;保坂卓臣;佐々木崇光;吉成浩一―;Matsubara K et al.
许多人对环境中甲状腺激素干扰化学品可能产生的不良影响表示担忧。新生儿期甲状腺激素的破坏可能导致对甲状腺激素稳态的永久性影响以及相关的发育障碍，因为甲状腺激素对于调节许多组织的生长和分化至关重要。为了了解一般新生儿给予甲状腺激素样化学物质对基因表达的长期改变，我们鉴定了新生儿暴露于三碘甲状腺原氨酸（T3）时肝脏（甲状腺激素轴的重要组成部分）中表达改变的基因。T3在出生后第1、3和5天（第0周）给予雄性F344大鼠。在8周大时，使用cDNA微阵列分析来识别因新生儿暴露于T3而改变表达的肝脏基因。在已确定的上调基因中，Olr59，Ethe1和Slc10a2的表达在暴露于T3的大鼠中特异性增加。有趣的是，当给予能够与TR结合的羟基化多溴联苯醚（PBDE）OH‐ BDE 42时，这些基因的肝脏表达改变确实增加。我们的数据表明，新生儿暴露于甲状腺激素可能会影响基因的长期表达，这可能是甲状腺激素干扰化学品对新生儿影响的有用标志物。版权所有© 2017约翰威利父子有限公司
Many concerns have been expressed regarding the possible adverse effects of thyroid hormone‐disrupting chemicals in the environment. The disruption of thyroid hormones in the neonatal period may lead to permanent effects on thyroid hormone homeostasis as well as related developmental disorders, as thyroid hormones are essential for regulating the growth and differentiation of many tissues. To understand the long‐term alteration in gene expressions by neonatal administration of thyroid hormone‐like chemicals in general, we identified genes whose expression was altered in the liver, an important component of the thyroid hormone axis, by neonatal exposure to triiodothyronine (T3). T3 was administered to male F344 rats on postnatal days 1, 3, and 5 (week 0). At 8 weeks of age, cDNA microarray analysis was used to identify hepatic genes whose expression was altered by neonatal exposure to T3. Among the up‐regulated genes that were identified, the expression ofOlr59,Ethe1, andSlc10a2increased specifically in rats neonatally exposed to T3. Interestingly, altered hepatic expression of these genes indeed increased when a hydroxylated polybrominated diphenyl ether (PBDE), OH‐BDE42, which is capable of binding to the TR, was given neonatally. Our data demonstrated that neonatal exposure to thyroid hormones could affect the long‐term expression of the genes, which could be useful markers for neonatal effects by thyroid hormone‐disrupting chemicals. Copyright © 2017 John Wiley & Sons, Ltd.