Expression and localization of alpha v integrins and their ligand vitronectin in normal ovarian epithelium and in ovarian carcinoma
Expression and localization of alpha v integrins and their ligand vitronectin in normal ovarian epithelium and in ovarian carcinoma
复制标题
DOI：
10.1006/gyno.1996.0225
复制
发表时间：
1996-08-01
影响因子：
4.7
通讯作者：
Gauduchon, P
中科院分区：
文献类型：
--
作者：
Carreiras, F;Denoux, Y;Gauduchon, P
在先前体外研究的扩展中[Carreiras等人，Int，J. Cancer 63，530-536（1995）]，并且为了理解上皮性卵巢肿瘤内由整联蛋白介导的粘附相互作用，比较研究了在不同分化阶段的卵巢癌和正常卵巢上皮中α v和β 3亚基以及玻连蛋白（Vn）的存在。通过免疫荧光法，分析了冷冻切片中α v（34例）、β 3（19例）和Vn（29例）的表达。α v在正常上皮和高度分化的肿瘤以及大多数中分化和低分化的癌中表达，具有相同的染色模式。β_3亚单位和Vn在正常卵巢上皮和高分化卵巢癌中也有表达，但在低分化卵巢癌中大多数无表达。同时检测α_3、β_3和Vn的病例分析表明，大部分正常卵巢上皮和高分化卵巢癌同时表达α_3、β_3和Vn;与此相反，在所有中、低分化癌中，β_3或Vn均不存在，本文讨论了α v β_3/Vn系统在卵巢上皮功能中的潜在作用，并推测该系统在卵巢癌中的改变可能有助于肿瘤的进展。(C)出版社：Academic Press，Inc.
In an extension of a previous in vitro study [Carreiras et al., Int, J. Cancer 63, 530-536 (1995)] and in an effort to understand the adhesive interactions mediated by integrins within epithelial ovarian tumors, the presence of the alpha v and beta 3 subunits and that of vitronectin (Vn) in ovarian carcinomas at various stages of differentiation and in normal ovarian epithelium were comparatively investigated, The study was performed on material from 34 patients, By immunofluorescence, cryostat sections were analyzed for their expression of alpha v (34 cases), beta 3 (19 cases), and Vn (29 cases). alpha v was expressed in normal epithelium and in highly differentiated tumors as well as in a majority of moderately and poorly differentiated carcinomas with identical staining pattern. beta 3 subunit and Vn were also expressed in normal cases and highly differentiated carcinomas, However, they were lacking in most of the less differentiated tumors, The analysis of cases which were simultaneously tested for the presence of alpha v, beta 3, and Vn revealed that a large proportion of normal ovarian epithelium and highly differentiated rumors simultaneously expressed alpha v, beta 3, and Vn; in contrast, in all moderately and poorly differentiated carcinomas either beta 3 or Vn was absent; The potential role of the alpha v beta 3/Vn system in ovarian epithelium functions is discussed, It is also speculated that modifications of this system in ovarian carcinomas might contribute to tumor progression. (C) 1996 Academic Press, Inc.