Repeatable target localization for long-term in vivo imaging of mice with 2-photon microscopy
Repeatable target localization for long-term in vivo imaging of mice with 2-photon microscopy
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DOI：
10.1016/j.jneumeth.2011.10.029
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发表时间：
2012-04-15
影响因子：
3
通讯作者：
Liebig, Christian
中科院分区：
文献类型：
--
作者：
Hefendehl, Jasmin K.;Milford, David;Liebig, Christian
小鼠体内重复成像已成为研究大脑结构和功能动态变化不可或缺的工具。我们描述了一种头部固定系统，它可以快速重新定位大脑内先前成像的感兴趣区域（ROI）。此类 ROI 可以在数周至数月内自动重新定位和成像，旋转变化可以忽略不计，只有很小的平移误差。先前存储的成像位置可以在几秒钟内完全自动重新定位。这种自动快速准确的重新定位简化了纵向成像实验中的图像采集和后处理。此外，由于激光仅用于数据采集，而不是用于寻找先前成像的 ROI，因此激光引起的组织损伤和光漂白的风险大大降低。因此，这里描述的头部固定装置似乎非常适合啮齿动物大脑的体内重复长期成像。 (C) 2011 Elsevier B.V. 保留所有权利。
Repetitive in vivo imaging in mice has become an indispensable tool for studying dynamic changes in structure and function of the brain. We describe a head fixation system, which allows rapid re-localization of previously imaged regions of interest (ROIs) within the brain. Such ROIs can be automatically relocated and imaged over weeks to months with negligible rotational change and only minor translational errors. Previously stored imaging positions can be fully automated re-localized within a few seconds. This automated rapid and accurate relocation simplifies image acquisition and post-processing in longitudinal imaging experiments. Moreover, as the laser is only used for data acquisition and not for finding previously imaged ROIs, the risk of laser induced tissue damage and photobleaching is greatly reduced. Thus, here described head fixation device appears well suited for in vivo repetitive long-term imaging in rodent brain. (C) 2011 Elsevier B.V. All rights reserved.